Human ABCB1 / MDR1 / P Glycoprotein

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

JSB-1

Unconjugated

Tissue culture supernatant

Unmodified

The antibody JSB-1 reacts with a conserved cytoplasmic epitope of the plasma membrane-associated 170-180 kD glycoprotein, the expression of which is strongly correlated with the degree of multi-drug-resistance (MDR) derived MDR cell lines and human MDR cell lines, including cell lines derived from lung, ovaries and B cell lymphomas. Cross-reacts with Chinese hamster. No cross-reactivity with mouse or rat.

• IHC
• IHC - Paraffin (1:50)
• IHC - Frozen
• Western blot
• Flow Cytometry

JSB-1 has potential value for diagnostic detection of MDR cells in human tumor samples. Working dilutions. Flow cytometry: at least 1:10. Western blotting: start optimizing working dilution a 1:10. Immunocytochemistry: acetone fixed cell preparation at least 1:20. Immunohistochemistry: acetone-fixed frozen sections at least 1:20, formalin-fixed paraffin embedded tissues 1:20. Instructions for use Optimal staining results are obtained with routine 2-step ABC or APAAP methods using acetone-fixed cytocentrifuge preparations or cryostat sections. In case the B-5 fixative is used (see below) also paraffin embedded tissue can be stained with the antibody. In Flow cytometry fixate cells in 10% (v/v) Lysing solution followed by primary antibody and antiMouse-FITC. In developing a Western blot staining pattern use an anti-Mouse-HRP.

Tissue Culture Supernatant, 0.1% Sodium Azide, 1% BSA

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08183 NCBI: NM_000927 NP_000918.2