Hamster ABCB1 / MDR1 / P Glycoprotein

Mouse

Hamster, Human, Mouse, Rat (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

C219

Unconjugated

Protein A purified

Unmodified

SDS-solubilized plasma membranes of an MDR (multidrug resistant) Chinese hamster ovary (CHO) cell line and a human cell line.

Recognizes an internal, highly conserved amino acid sequence (VQEALD and VQAALD) found in both protein isoforms, P-glycoprotein and MDR3 P-glycoprotein; not species specific. Species Crossreactivity: human, mouse, rat and hamster. Crossreactivity Proteins: a ~200kD protein which migrates in the same position as myosin and with C-erbB2 protein (p185c-erbB2).

• IHC
• IHC - Paraffin
• IHC - Frozen
• ICC
• Western blot (1:25 - 1:50)
• Immunoprecipitation
• Flow Cytometry

Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Flow Cytometry: Cell permeabilization required. Western Blot: 1:25-1:50. Immunohistochemistry (Paraffin and Frozen): For paraffin sections, dilute 1:12.5 for biotin based detection. The antibody should be incubated for 60 minutes at RT. Immunocytochemistry: Cytospin preparations. Positive control: Cell lines: Appropriate drug-sensitive parental cell lines and their multidrug resistant derivatives prepared by the same methods as the test specimens can be used as control materials.

PBS, 0.1% Sodium Azide, 1% BSA

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P08183 NCBI: NM_000927 NP_000918.2