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Catalog Number Size Price
LS-C744756-100 100 µg (1 mg/ml) $492 
A2M / Alpha-2-Macroglobulin Antibody - Western Blot of Biotin Conjugated Goat anti-Alpha-2-Macroglobulin Antibody. Lane 1: Alpha-2-Macroglobulin. Load: 100 ng per lane. Primary antibody: Alpha-2-Macroglobulin biotin conjugated antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.
A2M / Alpha-2-Macroglobulin Antibody - Goat anti Alpha-2-Macroglobulin antibody was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat. Images were collected using the BioRad VersaDoc System.
A2M / Alpha-2-Macroglobulin Antibody - Western Blot of Biotin Conjugated Goat anti-Alpha-2-Macroglobulin Antibody. Lane 1: Alpha-2-Macroglobulin. Load: 100 ng per lane. Primary antibody: Alpha-2-Macroglobulin biotin conjugated antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.
A2M / Alpha-2-Macroglobulin Antibody - Goat anti Alpha-2-Macroglobulin antibody was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody. Detection shown was using Dylight 649 conjugated Donkey anti goat. Images were collected using the BioRad VersaDoc System.
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Polyclonal Goat anti‑Human A2M / Alpha‑2‑Macroglobulin Antibody (Lyophilized, Biotin, WB) LS‑C744756

Polyclonal Goat anti‑Human A2M / Alpha‑2‑Macroglobulin Antibody (Lyophilized, Biotin, WB) LS‑C744756

Antibody:
A2M / Alpha-2-Macroglobulin Goat anti-Human Polyclonal (Lyophilized) (Biotin) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Biotin, Lyophilized
Price
Catalog Number
$492
LS-C744756-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
A2M / Alpha-2-Macroglobulin Goat anti-Human Polyclonal (Lyophilized) (Biotin) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Biotin, Lyophilized

Specifications

Description
Alpha-2-Macroglobulin antibody LS-C744756 is a biotin-conjugated goat polyclonal antibody to human Alpha-2-Macroglobulin (A2M). Validated for ELISA and WB.
Target
Human A2M / Alpha-2-Macroglobulin
Synonyms
A2M | CPAMD5 | A2MD | FWP007 | Alpha-2-M | Alpha-2-macroglobulin | S863-7
Host
Goat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Alpha-2-Macroglobulin [Human Plasma].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum as well as purified and partially purified alpha-2-MACROGLOBULIN [Human Plasma]. Cross reactivity against alpha-2-MACROGLOBULIN from other sources is unknown.
Applications
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:20000 - 1:100000)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About A2M / Alpha-2-Macroglobulin
P01023 NM_000014 NP_000005.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021