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Antibodies > A2M / Alpha-2-Macroglobulin > LS-C355390
 
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Catalog Number Size Price
LS-C355390-1 1 mg $857 
A2M / Alpha-2-Macroglobulin Antibody - Western blot analysis of Alpha2-Macroglobulin antibody was performed by loading 1000ng, 500ng, 250ng and 125ng of a Human Alpha2-Macroglobulin Recombinant protein and 5ul PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter and blocked with 5% BSA in TBST for at least 1 hour at room temperature. Alpha 2 Macroglobulin was detected at ~180kD using an Alpha2-Macroglobulin monoclonal antibody at a concentration of 2 µg/mL in 5% BSA in TBST overnight at 4C on a rocking platform, followed by an incubation with a goat anti-mouse IgG-HRP Superclonal secondary antibody at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.
A2M / Alpha-2-Macroglobulin Antibody - Western blot analysis of Alpha2-Macroglobulin antibody was performed by loading 5ul of Human Plasma and 5ul PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter and blocked with 5% milk in TBST for at least 1 hour at room temperature. Alpha 2 Macroglobulin was detected at ~180kD using an Alpha2-Macroglobulin monoclonal antibody at a concentration of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform, followed by an incubation with a goat anti-mouse IgG-HRP Superclonal secondary antibody at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.
A2M / Alpha-2-Macroglobulin Antibody - Direct ELISA analysis of Alpha2-Macroglobulin was performed by coating wells of a 96-well plate with 100ul per well of Alpha2- Macroglobulin recombinant protein diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4C. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 100ul per well of a mouse Alpha2-Macroglobulin monoclonal antibody starting at a concentration of 2 µg/mL and serially diluting it to a concentration of 0.03125 µg/mL for 2 hours at room temperature. The plate was washed and incubated with 100ul per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 30 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid and absorbances were read on a spectrophotometer at 450-550 nm.
A2M / Alpha-2-Macroglobulin Antibody - Western blot analysis of Alpha2-Macroglobulin antibody was performed by loading 1000ng, 500ng, 250ng and 125ng of a Human Alpha2-Macroglobulin Recombinant protein and 5ul PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter and blocked with 5% BSA in TBST for at least 1 hour at room temperature. Alpha 2 Macroglobulin was detected at ~180kD using an Alpha2-Macroglobulin monoclonal antibody at a concentration of 2 µg/mL in 5% BSA in TBST overnight at 4C on a rocking platform, followed by an incubation with a goat anti-mouse IgG-HRP Superclonal secondary antibody at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura.
A2M / Alpha-2-Macroglobulin Antibody - Western blot analysis of Alpha2-Macroglobulin antibody was performed by loading 5ul of Human Plasma and 5ul PageRuler Plus Prestained Protein Ladder onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter and blocked with 5% milk in TBST for at least 1 hour at room temperature. Alpha 2 Macroglobulin was detected at ~180kD using an Alpha2-Macroglobulin monoclonal antibody at a concentration of 1 µg/mL in 5% milk in TBST overnight at 4C on a rocking platform, followed by an incubation with a goat anti-mouse IgG-HRP Superclonal secondary antibody at a dilution of 1:1000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico.
A2M / Alpha-2-Macroglobulin Antibody - Direct ELISA analysis of Alpha2-Macroglobulin was performed by coating wells of a 96-well plate with 100ul per well of Alpha2- Macroglobulin recombinant protein diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4C. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 100ul per well of a mouse Alpha2-Macroglobulin monoclonal antibody starting at a concentration of 2 µg/mL and serially diluting it to a concentration of 0.03125 µg/mL for 2 hours at room temperature. The plate was washed and incubated with 100ul per well of an HRP-conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 30 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid and absorbances were read on a spectrophotometer at 450-550 nm.
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Monoclonal Mouse anti‑Human A2M / Alpha‑2‑Macroglobulin Antibody (clone F1‑P1C11 #3, WB) LS‑C355390

Monoclonal Mouse anti‑Human A2M / Alpha‑2‑Macroglobulin Antibody (clone F1‑P1C11 #3, WB) LS‑C355390

Submit Review LSBio Guarantee
Antibody:
A2M / Alpha-2-Macroglobulin Mouse anti-Human Monoclonal (F1-P1C11 #3) Antibody
Application:
WB, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$857
LS-C355390-1
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
A2M / Alpha-2-Macroglobulin Mouse anti-Human Monoclonal (F1-P1C11 #3) Antibody
Application:
WB, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Alpha-2-Macroglobulin antibody LS-C355390 is an unconjugated mouse monoclonal antibody to human Alpha-2-Macroglobulin (A2M). Validated for ELISA, IP, RIA and WB.
Target
Human A2M / Alpha-2-Macroglobulin
Synonyms
A2M | CPAMD5 | A2MD | FWP007 | Alpha-2-M | Alpha-2-macroglobulin | S863-7
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
F1-P1C11 #3
Conjugations
Unconjugated
Purification
Ion exchange chromatography
Modifications
Unmodified
Immunogen
Purified human plasma AMG.
Specificity
The antibody targets Alpha2-Macroglobulin in ELISA, immunoprecipitation, and radioimmunoassay applications and shows reactivity with Human samples. It detects Alpha2-Macroglobulin which has a predicted molecular weight of approximately 162 kD.
Applications
  • Western blot
  • Immunoprecipitation
  • ELISA
  • Radioimmunoassay
Presentation
0.015 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.1% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About A2M / Alpha-2-Macroglobulin
P01023 NM_000014 NP_000005.2

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See all A2M / Alpha-2-Macroglobulin Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/18/2024
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