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VPS35

Vacuolar protein sorting-associated protein 35

VPS35 belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Gene Name: Vacuolar protein sorting-associated protein 35
Synonyms: VPS35, MEM3, Maternal-embryonic 3, Vesicle protein sorting 35, PARK17, HVPS35
Target Sequences: NM_018206 NP_060676.2 Q96QK1

Publications (2)

1
Rab GTPase-activating proteins in autophagy: regulation of endocytic and autophagy pathways by direct binding to human ATG8 modifiers. Popovic D, Akutsu M, Novak I, Harper JW, Behrends C, Dikic I. Molecular and cellular biology. 2012 32:1733-44. (WB; Human) [Full Text Article] [PubMed:22354992] [PMC:PMC3347240] Related Antibodies: LS-B5909.
2
Novel Insights into the Cellular Localization and Regulation of the Autophagosomal Proteins LC3A, LC3B and LC3C. Marius W Baeken, Katja Weckmann, Philip Diefenthäler, Jan Schulte, Kamran Yusifli, Bernd Moosmann, Christian Behl, Parvana Hajieva. Cells. 2020 October;9: [Full Text Article] [PubMed:33081014] [PMC:PMC7603224] Related Antibodies: LS-B5909.

☰ Filters
Antibodies (19)
Proteins (5)
Over-Expression Lysate (2)
Primary (19)
Recombinant (3)
VPS35 (24)
Human (17)
Mouse (14)
Rat (9)
Bovine (4)
Dog (3)
Hamster (1)
Horse (4)
Monkey (1)
Pig (3)
Rabbit (4)
IHC (8)
IHC-P (5)
WB (14)
ELISA (6)
ICC (1)
IF (1)
IP (1)
Peptide-ELISA (2)
rabbit (18)
goat (1)
Product Group
IHCPlus (1)
PathPlus Neuro (1)
IgG (11)
polyclonal pc (19)
Biotin Conjugated (1)
FITC Conjugated (1)
HRP Conjugated (1)
Unconjugated (16)
C-Terminus (4)
Internal Region (1)
aa190-240 (1)
aa733-796 (1)
aa746-796 (1)
aa783-796 (1)
No (23)
Yes (1)
10His, N-terminus + Myc, C-terminus (1)
GST (1)
GST, N-terminus (1)
Myc-DDK (Flag) (2)
Human (4)
293T Cells (1)
E. coli (1)
HEK 293 Cells (1)
Wheat Germ Extract (2)
Purified (2)
VPS35 Antibody - Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
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Neuroscience
VPS35 Goat anti-Human Polyclonal (aa783-796) Antibody
Rabbit, Mouse, Bovine, Rat, Hamster, Horse, Human, Monkey
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µg/$485
VPS35 Antibody - VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS35 antibody [C3], C-term dilution:1:250.
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VPS35 Rabbit anti-Human Polyclonal (aa733-796) Antibody
Human
ICC, IHC, IHC-P, WB
Unconjugated
100 µl/$450
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VPS35 Rabbit anti-Human Polyclonal (aa190-240) Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
500 µg/$393
VPS35 Antibody - Immunohistochemistry of paraffin-embedded Human brain tissue  using VPS35 Polyclonal Antibody at dilution of 1:25(×200)
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VPS35 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IHC, WB
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
VPS35 Antibody - Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue  using VPS35 Polyclonal Antibody at dilution of 1:25(×200)
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VPS35 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IHC, WB
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
VPS35 Antibody - Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE) HIER using 10 mM sodium citrate buffer pH 6.0
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Fast Shipping
VPS35 Rabbit anti-Human Polyclonal (Internal Region) Antibody
IHC-P
Unconjugated
50 µg/$460
VPS35 Antibody - Immunohistochemistry of paraffin-embedded human ovarian cancer at dilution 1:100
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VPS35 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P
Unconjugated
50 µl/$294; 100 µl/$360
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VPS35 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IHC
Unconjugated
20 µl/$254; 60 µl/$296; 120 µl/$355; 200 µl/$450
VPS35 Antibody - Western blot analysis of extracts of various cell lines.
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VPS35 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
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VPS35 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
100 µg/$357
VPS35 Antibody - Western Blot (WB) analysis of HeLa cells using VPS35 Polyclonal Antibody diluted at 1:500.
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VPS35 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
VPS35 Antibody - Detection of human and mouse VPS35 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-VPS35 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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VPS35 Rabbit anti-Human Polyclonal (aa746-796) Antibody
Mouse, Human
IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
VPS35 Antibody - Western blot analysis of extracts of HepG2 cells using VPS35 antibody. The lane on the left was treated with blocking peptide.
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VPS35 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
VPS35 Antibody - Western blot of Vps35 Antibody - C-terminal region in Mouse Lung cells lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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VPS35 Rabbit anti-Mouse Polyclonal (C-Terminus) Antibody
Mouse
WB
Unconjugated
100 µl/$424
VPS35 Antibody - Western blot of Vps35 Antibody - C-terminal region in Mouse Lung cells lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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VPS35 Rabbit anti-Mouse Polyclonal (C-Terminus) (HRP) Antibody
Rabbit, Mouse, Dog, Bovine, Rat, Pig, Horse, Human
WB
HRP Conjugated
100 µl/$469
VPS35 Antibody - Western blot of Vps35 Antibody - C-terminal region in Mouse Lung cells lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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VPS35 Rabbit anti-Mouse Polyclonal (C-Terminus) (Biotin) Antibody
Rabbit, Mouse, Dog, Bovine, Rat, Pig, Horse, Human
WB
Biotin Conjugated
100 µl/$469
VPS35 Antibody - Western blot of Vps35 Antibody - C-terminal region in Mouse Lung cells lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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VPS35 Rabbit anti-Mouse Polyclonal (C-Terminus) (FITC) Antibody
Rabbit, Mouse, Dog, Bovine, Rat, Pig, Horse, Human
WB
FITC Conjugated
100 µl/$469
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VPS35 Rabbit anti-Human Polyclonal Antibody
Human
Unconjugated
100 µg/$341
VPS35 Antibody - Immunochemical staining of human VPS35 in human duodenum with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
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VPS35 Rabbit anti-Human Polyclonal Antibody
Human
IHC-P
Unconjugated
100 µl/$412
VPS35 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
VPS35 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
91.5 kDa
20 µg/$150
VPS35 Protein - 12.5% SDS-PAGE of human VPS35 stained with Coomassie Blue
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Wheat Germ Extract
GST
118.1 kDa
10 µg/$479; 25 µg/$670
VPS35 Protein - Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of Recombinant Human Vacuolar protein sorting-associated protein 35(VPS35) could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) VPS35.
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E. coli
10His, N-terminus + Myc, C-terminus
96.7 kDa
1 mg/$1,679; 100 µg/$500; 20 µg/$364
VPS35 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
91.5 kDa
100 µg/$494
Viewing 1-24 of 24 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).