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UBE2M / UBC12

ubiquitin-conjugating enzyme E2M

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.

Gene Name: ubiquitin-conjugating enzyme E2M
Synonyms: UBE2M, NEDD8 protein ligase, NEDD8 carrier protein, NEDD8-conjugating enzyme Ubc12, HUbc12, UBC-RS2, UBC12, Ubiquitin-protein ligase M, Yeast UBC12 homolog, Ubiquitin carrier protein M
Target Sequences: NM_003969 NP_003960.1 P61081

☰ Filters
Antibodies (13)
Proteins (7)
Over-Expression Lysate (2)
Primary (13)
Recombinant (5)
UBE2M / UBC12 (20)
Human (13)
Mouse (8)
Rat (4)
Bovine (3)
Chimpanzee (3)
Dog (5)
Guinea pig (3)
Horse (3)
Monkey (5)
Rabbit (3)
Xenopus (3)
IHC (1)
WB (13)
ELISA (2)
IF (3)
IP (4)
Peptide-ELISA (1)
rabbit (11)
mouse (2)
IgG (9)
IgG1 (2)
monoclonal mc (2)
polyclonal pc (11)
OTI2D9 (2)
Biotin Conjugated (1)
Carrier-free (1)
FITC Conjugated (1)
HRP Conjugated (1)
Unconjugated (10)
N-Terminus (4)
No (20)
GST (1)
His (1)
Myc-DDK (Flag) (2)
Human (6)
293T Cells (1)
E. coli (4)
HEK 293 Cells (1)
Wheat Germ Extract (1)
Purified (2)
Low endotoxin level (2)
UBE2M / UBC12 Protein
Select
E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
Select
UBE2M / UBC12 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IP, WB
Unconjugated
100 µg/$341
UBE2M / UBC12 Antibody - Anti-UBE2M mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2M.
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UBE2M / UBC12 Mouse anti-Human Monoclonal (OTI2D9) Antibody
Mouse, Dog, Human, Monkey
IF, WB
Unconjugated
100 µl/$434
UBE2M / UBC12 Antibody - Anti-UBE2M mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY UBE2M.
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UBE2M / UBC12 Mouse anti-Human Monoclonal (Carrier-free) (OTI2D9) Antibody
Mouse, Dog, Human, Monkey
IF, WB
Unconjugated, Carrier-free
100 µg/$511
UBE2M / UBC12 Antibody - 1:100 staining mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
Select
UBE2M / UBC12 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
Select
UBE2M / UBC12 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IP, WB
Unconjugated
100 µl/$412
Select
UBE2M / UBC12 Rabbit anti-Human Polyclonal Antibody
Human
IP, WB
Unconjugated
100 µl/$412
UBE2M / UBC12 Antibody - Anti-UBE2M rabbit polyclonal antibody at 1:500 dilution. Lane A: 293T Whole Cell Lysate. Lane B: HEK-293 Whole Cell Lysate. Lane C: Jurkat Whole Cell Lysate. Lane D: U251MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 21 kDa.
Select
UBE2M / UBC12 Rabbit anti-Human Polyclonal Antibody
Human
IP, WB
Unconjugated
100 µl/$412
UBE2M / UBC12 Antibody - 721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
UBE2M / UBC12 Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Mouse, Human
WB
Unconjugated
100 µl/$424
UBE2M / UBC12 Antibody - 721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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UBE2M / UBC12 Rabbit anti-Human Polyclonal (N-Terminus) (HRP) Antibody
Xenopus, Horse, Rat, Rabbit, Mouse, Dog, Guinea pig, Bovine, Human, Monkey, Chimpanzee
WB
HRP Conjugated
100 µl/$469
UBE2M / UBC12 Antibody - 721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
UBE2M / UBC12 Rabbit anti-Human Polyclonal (N-Terminus) (Biotin) Antibody
Xenopus, Horse, Rat, Rabbit, Mouse, Dog, Guinea pig, Bovine, Human, Monkey, Chimpanzee
WB
Biotin Conjugated
100 µl/$469
UBE2M / UBC12 Antibody - 721_B cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
UBE2M / UBC12 Rabbit anti-Human Polyclonal (N-Terminus) (FITC) Antibody
Xenopus, Horse, Rat, Rabbit, Mouse, Dog, Guinea pig, Bovine, Human, Monkey, Chimpanzee
WB
FITC Conjugated
100 µl/$469
UBE2M / UBC12 Antibody - Western blot analysis of extracts of various cells.
Select
UBE2M / UBC12 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
UBE2M / UBC12 Antibody - Anti-UBE2M rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa.
Select
UBE2M / UBC12 Rabbit anti-Human Polyclonal Antibody
Human
WB
Unconjugated
100 µl/$412
Select
E. coli
None
Less than 1.0 EU/µg protein (determined by LAL method).
20.9kD
10 µg/$233; 50 µg/$277; 500 µg/$725; 1 mg/$1,113
UBE2M / UBC12 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
20.7 kDa
20 µg/$150
UBE2M / UBC12 Protein - 12.5% SDS-PAGE of human UBE2M stained with Coomassie Blue
Select
Wheat Germ Extract
GST
45.87 kDa
10 µg/$479; 25 µg/$670
Select
E. coli
None
21 kDa
100 µg/$661
Select
E. coli
None
Less than 1.0 EU/µg protein (determined by LAL method).
20.9 kDa
10 µg/$322; 50 µg/$365
UBE2M / UBC12 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
20.7 kDa
100 µg/$494
Viewing 1-20 of 20 product results



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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).