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Rabbit Anti-Mouse IgG Secondary Antibody (AP) LS-C60794

Ordering

Wt. Vol. Conc. Price
1000 µg - - $285

LSBio (Direct) LSBio (Direct)
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Mouse IgG Antibody LS-C60794 Images

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Western Blot
Western Blot of Alkaline Phosphatase Conjugated Rabbit anti-Mouse IgG antibody.

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Rabbit Polyclonal to Mouse IgG
Mouse
IHC, Western blot, ELISA
AP Conjugated

Details

Mouse IgG
Rabbit
Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Alkaline Phosphatase (AP)
Immunoaffinity purified

Applications

IHC, Western blot, ELISA (1:4000 - 1:16000)

Specificity and Use

Mouse IgG antibody was raised against mouse IgG1 heavy chain.
anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1 % cross reactivity against other mouse heavy or light chain isotypes
Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate catalog no. NPP-10 as a substrate for 30 minutes at room temperature.

Packaging

0.05 M Tris chloride, 0.15 M sodium chloride, 0.001 M magnesium chloride, 0.0001 M zinc chloride, 50% glycerol, pH 8.0, 10 mg/ml BSA (IgG, protease free), 0.01% sodium azide.
Store at 4°C. Do not freeze.
For research use only.

About Mouse IgG

Mouse Immunoglobulin G (IgG)

Western Blot

Western Blot
Western Blot of Alkaline Phosphatase Conjugated Rabbit anti-Mouse IgG antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 100 ng per lane. Primary antibody: none. Secondary antibody: Alkaline Phosphatase mouse secondary antibody at 1:1000 for 60 min at RT. Block: MB-070 for 30 min RT. Predicted/Observed size: 55 kDa, 28 kDa for Mouse IgG. Other band(s): none.
 

Requested From: United States
Date Requested: 10/24/2014

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