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Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $385

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
antibodies@lsbio.com
 

B7-H1 / PD-L1 / CD274 Antibody LS-B480 Images

Immunohistochemistry
Anti-PD-L1 antibody IHC staining of human thymus.

Anti-B7-H1 / PD-L1 / CD274 Antibody LS-B480 IHC-plus™

Rabbit anti-Human Polyclonal, Internal, Unconjugated

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Details

Applications

Human B7-H1 / PD-L1 / CD274
Rabbit
Human
Polyclonal
Immunoaffinity Chromatography
Unconjugated (Unconjugated)
IHC-P (5 µg/ml)
Western blot
Optimal dilution to be determined by the researcher.

Specificity and Use

B7-H1 / PD-L1 / CD274 antibody was raised against synthetic peptide from human CD274.
Internal
17 amino acid peptide from near the center of human PDL-1.
Immunohistochemistry: LS-B480 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B480 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About B7-H1 / PD-L1 / CD274

Q9NZQ7 NM_014143 NP_054862.1

CD274 Antibody, B7-H Antibody, B7 homolog 1 Antibody, B7-H1 Antibody, B7H1 Antibody, CD274 molecule Antibody, CD274 antigen Antibody, PDCD1 ligand 1 Antibody, PDL1 Antibody, PD-L1 Antibody, PDCD1L1 Antibody, PDCD1LG1 Antibody, Programmed death ligand 1 Antibody

CD274, also known as B7-H1 and PD-1L, a cell surface glycoprotein, is a member of the B7 family of co-stimulatory molecules.


B7-H1 / PD-L1 / CD274 Antibodies (25)

Target Host Clonality Format Epitope Reactivity Applications
B7-H1 / PD-L1 / CD... Rabbit Polyclonal Human IHC-P, WB
B7-H1 / PD-L1 / CD274 Antibody LS-B480 Rabbit Polyclonal thumb(1)
50 µg - 385.00
Applications: IHC-P, WB Epitope: Internal  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Goat Polyclonal Human ELISA, IHC-P, WB
B7-H1 / PD-L1 / CD274 Antibody LS-B2999 Goat Polyclonal thumb(3)
50 µg - 385.00
Applications: IHC-P, WB, ELISA Epitope: C-Terminus  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human ELISA, Flo
B7-H1 / PD-L1 / CD274 Antibody [130021] LS-C128031 Mouse Monoclonal
100 µg - 455.00
Applications: Flo, ELISA Epitope: aa19-338  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Rabbit Polyclonal Human WB
B7-H1 / PD-L1 / CD274 Antibody LS-C146478 Rabbit Polyclonal thumb(1)
50 µg - 380.00
Applications: WB Epitope: C-Terminus  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Rat Monoclonal Mouse Flo
B7-H1 / PD-L1 / CD274 Antibody [MIH6] LS-C58198 Rat Monoclonal thumb(1)
100 µg - 325.00
Applications: Flo Epitope:  
Reactivity: Mu Format: FITC  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human ELISA, Flo
B7-H1 / PD-L1 / CD274 Antibody [ANC6H1] LS-C134636 Mouse Monoclonal
100 µg - 340.00
Applications: Flo, ELISA Epitope:  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human ELISA, Flo
B7-H1 / PD-L1 / CD274 Antibody [ANC6H1] LS-C134637 Mouse Monoclonal
100 µg - 430.00
Applications: Flo, ELISA Epitope:  
Reactivity: Hu Format: Biotin  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human ELISA, Flo
B7-H1 / PD-L1 / CD274 Antibody [ANC6H1] LS-C134638 Mouse Monoclonal
100 µg - 340.00
Applications: Flo, ELISA Epitope:  
Reactivity: Hu Format: Azide-free  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human Flo
B7-H1 / PD-L1 / CD274 Antibody LS-C128033 Mouse Monoclonal
100 µg - 425.00
Applications: Flo Epitope:  
Reactivity: Hu Format: FITC  
B7-H1 / PD-L1 / CD... Rat Monoclonal Mouse Flo, IHC-Fr, IP
B7-H1 / PD-L1 / CD274 Antibody [MIH6] LS-C58199 Rat Monoclonal thumb(1)
100 µg - 225.00
Applications: IHC-Fr, Flo, IP Epitope:  
Reactivity: Mu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human Flo, IHC-P
B7-H1 / PD-L1 / CD274 Antibody [27A2] LS-C179481 Mouse Monoclonal thumb(1)
100 µg - 340.00
Applications: IHC-P, Flo Epitope:  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Goat Polyclonal Mouse ELISA, Flo, IHC, WB
B7-H1 / PD-L1 / CD274 Antibody LS-C150153 Goat Polyclonal
100 µg - 395.00
Applications: IHC, WB, Flo... Epitope:  
Reactivity: Mu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Rat Monoclonal Mouse Flo, IHC-Fr, IP
B7-H1 / PD-L1 / CD274 Antibody LS-C123195 Rat Monoclonal
250 µg - 410.00
Applications: IHC-Fr, Flo, IP Epitope:  
Reactivity: Mu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Rat Monoclonal Mouse Flo, IHC-Fr, IP
B7-H1 / PD-L1 / CD274 Antibody LS-C123196 Rat Monoclonal
100 µg - 335.00
Applications: IHC-Fr, Flo, IP Epitope:  
Reactivity: Mu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Rat Monoclonal Mouse Flo
B7-H1 / PD-L1 / CD274 Antibody [1-111A] LS-C106660 Rat Monoclonal thumb(2)
50 µg - 240.00
100 µg - 310.00
500 µg - 465.00
Applications: Flo Epitope:  
Reactivity: Mu Format: Biotin  
B7-H1 / PD-L1 / CD... Rat Monoclonal Mouse Flo
B7-H1 / PD-L1 / CD274 Antibody [1-111A] LS-C107317 Rat Monoclonal thumb(1)
50 µg - 230.00
100 µg - 290.00
500 µg - 450.00
Applications: Flo Epitope:  
Reactivity: Mu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human Flo
B7-H1 / PD-L1 / CD274 Antibody [MIH2] LS-C58200 Mouse Monoclonal thumb(1)
100 µg - 255.00
Applications: Flo Epitope:  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human Flo
B7-H1 / PD-L1 / CD274 Antibody [29E.2A3] LS-C40827 Mouse Monoclonal thumb(1)
100 µg - 225.00
Applications: Flo Epitope:  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Rabbit Polyclonal Human WB
B7-H1 / PD-L1 / CD274 Antibody LS-C168531 Rabbit Polyclonal thumb(1)
100 µg - 290.00
Applications: WB Epitope: aa260-290  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Rabbit Polyclonal Human WB
B7-H1 / PD-L1 / CD274 Antibody LS-C169740 Rabbit Polyclonal thumb(1)
50 µg - 365.00
Applications: WB Epitope: C-Terminus  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human Flo
B7-H1 / PD-L1 / CD274 Antibody [MIH3] LS-C179365 Mouse Monoclonal thumb(1)
1000 µl - 355.00
Applications: Flo Epitope:  
Reactivity: Hu Format: Biotin  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human Flo
B7-H1 / PD-L1 / CD274 Antibody [27A2] LS-C179482 Mouse Monoclonal
50 tst - 340.00
Applications: Flo Epitope:  
Reactivity: Hu Format: PE  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human Flo
B7-H1 / PD-L1 / CD274 Antibody [MIH3] LS-C179364 Mouse Monoclonal
100 µg - 340.00
Applications: Flo Epitope:  
Reactivity: Hu Format: Unconjugated  
B7-H1 / PD-L1 / CD... Rat Monoclonal Mouse Flo
B7-H1 / PD-L1 / CD274 Antibody LS-C128034 Rat Monoclonal
100 µg - 335.00
Applications: Flo Epitope:  
Reactivity: Mu Format: FITC  
B7-H1 / PD-L1 / CD... Mouse Monoclonal Human Flo
B7-H1 / PD-L1 / CD274 Antibody LS-C125334 Mouse Monoclonal
100 tst - 435.00
Applications: Flo Epitope:  
Reactivity: Hu Format: PE  

Immunohistochemistry

Immunohistochemistry
Anti-PD-L1 antibody IHC staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B480 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 5/18/2013
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