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Anti-VPS35 Antibody (aa733-796) LS-C185941

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.93 mg/ml $335

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VPS35 Antibody LS-C185941 Images

[click image for a larger image and more details]
Immunohistochemistry
VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on colon carcinoma...
ICC
Immunofluorescence of methanol-fixed HCT116 using VPS35 antibody at 1:500 dilution.
Western Blot
Sample (30 ug of whole cell lysate).
Western Blot
Sample (50 ug of whole cell lysate).

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Rabbit Polyclonal to Human VPS35
Human
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human VPS35
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (1:100 - 1:1000), ICC (1:100 - 1:1000), Western blot (1:500 - 1:3000)

Specificity and Use

VPS35 antibody was raised against synthetic peptide corresponding to a region within amino acids 733 and 796 of VPS35 (SwissProt Q96QK1).
aa733-796
Human VPS35
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About VPS35

Q96QK1 NM_018206 NP_060676.2

VPS35 Antibody, MEM3 Antibody, Maternal-embryonic 3 Antibody, Vesicle protein sorting 35 Antibody, PARK17 Antibody, HVPS35 Antibody


Immunohistochemistry

Immunohistochemistry
VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS35 antibody [C3], C-term dilution:1:250.

ICC

ICC
Immunofluorescence of methanol-fixed HCT116 using VPS35 antibody at 1:500 dilution.

Western Blot

Western Blot
Sample (30 ug of whole cell lysate). A: H1299, B: HeLa S3 , C: Hep G2 , D: MOLT4 , E: Raji . 7.5% SDS PAGE. VPS35 antibody diluted at 1:500

Western Blot

Western Blot
Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. VPS35 antibody diluted at 1:1000.

Requested From: United States
Date Requested: 9/30/2014

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