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Catalog Number Size Price
LS-B9320-200 200 µl $460 
UCHL1 / PGP9.5 Antibody - Anti-UCHL1 / PGP9.5 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
UCHL1 / PGP9.5 Antibody - Fluorescent image of U251 cell stained with UCHL1 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with UCHL1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). UCHL1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
UCHL1 / PGP9.5 Antibody - UCHL1 Antibody western blot of U266,NCI-H1299,mouse Neuro-2a cell line and mouse brain tissue lysates (35 ug/lane). The UCHL1 antibody detected the UCHL1 protein (arrow).
UCHL1 / PGP9.5 Antibody - Western blot of lysates from SH-SY5Y cell line and mouse brain tissue (from left to right),using UCHL1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
UCHL1 / PGP9.5 Antibody - Anti-UCHL1 / PGP9.5 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
UCHL1 / PGP9.5 Antibody - Fluorescent image of U251 cell stained with UCHL1 Antibody. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with UCHL1 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). UCHL1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
UCHL1 / PGP9.5 Antibody - UCHL1 Antibody western blot of U266,NCI-H1299,mouse Neuro-2a cell line and mouse brain tissue lysates (35 ug/lane). The UCHL1 antibody detected the UCHL1 protein (arrow).
UCHL1 / PGP9.5 Antibody - Western blot of lysates from SH-SY5Y cell line and mouse brain tissue (from left to right),using UCHL1 Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
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IHC‑plus™ Polyclonal Rabbit anti‑Human UCHL1 / PGP9.5 Antibody (aa187‑214, IHC, IF, WB) LS‑B9320

IHC‑plus™ Polyclonal Rabbit anti‑Human UCHL1 / PGP9.5 Antibody (aa187‑214, IHC, IF, WB) LS‑B9320

Note: This antibody replaces LS-C160410
Antibody:
UCHL1 / PGP9.5 Rabbit anti-Human Polyclonal (aa187-214) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$460
LS-B9320-200
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
UCHL1 / PGP9.5 Rabbit anti-Human Polyclonal (aa187-214) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
PGP9.5 antibody LS-B9320 is an unconjugated rabbit polyclonal antibody to PGP9.5 (UCHL1) (aa187-214) from human. It is reactive with human and mouse. Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human UCHL1 / PGP9.5
Synonyms
UCHL1 | Ubiquitin C-terminal hydrolase | PARK5 | PGP9.5 | PGP95 | Ubiquitin thioesterase L1 | Neuron cytoplasmic protein 9.5 | PGP 9.5 | Uch-L1 | Ubiquitin carboxyl-terminal esterase L1 | HEL-117 | NDGOA | HEL-S-53 | SPG79
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Pig, Cow (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa187-214
Specificity
This UCHL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-214 amino acids from the C-terminal region of human UCHL1.
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About UCHL1 / PGP9.5
P09936 NM_004181 NP_004172.2

Validation

UCHL1 / PGP9.5 Antibody - Anti-UCHL1 / PGP9.5 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-UCHL1 / PGP9.5 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-UCHL1 / PGP9.5 antibody IHC staining of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

LSBio Ratings

IHC-plus™ UCHL1 / PGP9.5 Antibody (aa187-214) for IHC, IF/Immunofluorescence, WB/Western LS-B9320 has an LSBio Rating of
Laboratory Validation Score (4)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024