Human UCHL1 / PGP9.5

Rabbit

Human (tested or 100% immunogen sequence identity)

Rat, Bovine (at least 90% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with Biotin, DY650, DY488, DY550.

Immunoaffinity purified

Unmodified

A synthetic peptide made to an internal portion of the human UCHL1 protein sequence (between residues 100-200). UniProt P09936. Percent identity by BLAST analysis: Primate, Guinea (100%); Rat (99%); Bovine (92%).

aa100-200

Rabbit Polyclonal to PGP9.5 - Peripheral Nerve Fiber marker.

• IHC
• IHC - Paraffin (2.5 - 5 µg/ml)
• Western blot (0.5 - 2 µg/ml)

Immunohistochemistry: LS-B489 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B489 was determined to be 2.5-5 ug/ml.

Tris-Glycine, 150 mM NaCl, 0.05% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P09936 NCBI: NM_004181 NP_004172.2