Human TP73 / p73

Mouse

Human, Mouse (tested or 100% immunogen sequence identity)

IgG Monoclonal

5B429

Unconjugated

Protein G purified

Unmodified

Full-length human p73. It reacts with an epitope that is located on the N-Terminus of human p73. It does not cross react with p53.

N-Terminus

Recognizes all of the TAp73 isoforms i. e. isoforms with an intact NH2-terminal transactivation domain (Thottassery et el, 2006, PMID 16505115). Sayan et al 2005 (PMID 15781249) showed that p73 IMG-246/ NBP2-24737 reacted with all tested TAp73 isoforms but not deltaNp73 whereas p73 antibody clone 5B1288 (NB100-56674 / IMG-259A ) showed reactivity against all p73 isoforms including delta Np73. Caspase-cleaved p73 fragments, including 55 kD and 45 kD, have been described in literature (Sayan et al, 2008). p73 antibody clone 5B429 has also been referred to as 429 in the literature.

• IHC
• IHC - Paraffin (5 µg/ml)
• ICC
• Western blot (2 µg/ml)
• Immunoprecipitation

Immunohistochemistry: LS-B2086 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2086 was determined to be 5 ug/ml.

PBS, 0.05% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: O15350 NCBI: NM_005427 NP_005418.1