Human TP53 / p53

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Protein G purified

Unmodified

Gel-purified p53 containing aa 14-389 of human p53.

aa14-389

This antibody recognizes human, mouse, and dog p53.

• IHC - Paraffin (2 - 4 µg/ml)
• IHC - Frozen (2 - 4 µg/ml)
• Western blot (0.5 - 1 mg/ml)
• Immunoprecipitation (10 µg/ml)
• Flow Cytometry
• ELISA

ELISA, FACS, IHC (frozen and paraffin), Immunoprecipitation, Immunoblotting. IHC: use at 2-4ug/ml. Extensive washing may be necessary. This product does not require protein digestion or microwave antigen retrieval prior to staining. IP: use at 10ug/mg lysate. This antibody reacts with only MUTANT p53 protein under non-denaturing conditions. Immunoblotting: use at 0.5-1.0mg/ml. A band of 53kDa is detected. These are recommended concentrations. Enduser should determine optimal concentrations for their applications.

PBS, pH 7.4

Store at -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P04637 NCBI: NM_000546 NP_000537.3