Human TNF Alpha

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Antiserum

Unmodified

TNF alpha antibody used to produce this IgG fraction antibody was prepared by repeated immunizations with recombinant human TNFa.

This antibody is primarily directed against mature 17,000 MW human TNFa and is useful in determining its presence in various assays. In general, this antibody also detects primate TNFa in the same formats using similar dilutions. The antibody does not recognize human TNFß (lymphotoxin). This IgG fraction antibody will recognize the cell-bound precursor of TNFa as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of human and primate TNFa activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.

• IHC
• IHC - Paraffin
• Immunofluorescence
• Western blot (1:500 - 1:2000)
• ELISA (1:1000 - 1:5000)

This IgG fraction antibody of anti-Human TNFa has been tested for use in neutralizations, ELISA, immunohistochemistry and immunoblotting. It recognizes the 17000 MW TNFa. Reactivity in other immunoassays is unknown.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl

Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01375 NCBI: NM_000594 NP_000585.2