Human TGFB1 / TGF Beta 1

Chicken

Human, Mouse (tested or 100% immunogen sequence identity)

IgY Polyclonal

Biotin

Affinity purified

Unmodified

Recombinant human TGF-b1 expressed in CHO cells.

Recognizes human TGFb1. When used in combination with the monoclonal capture antibody LS-C10854 in a sandwich ELISA, ~5% cross-reactivity with TGF-b1.2 and 1% cross-reactivity with rhTGF-b2, rcTGF-b3 and raTG-b5 was observed.

• Western blot (0.1 - 0.2 µg/ml)
• ELISA

Suitable for use in ELISA and Western Blot. Plates are coated with 100 ul/well of the capture antibody in combination with 100 ul/well of the detection antibody at 300 ng/ml. An ELISA for sample volumes of 100 ul can be obtained. Titrate each preparation of the recombinant protein for standard preparation to arrive at the most suitable dose range. For this antibody pair, a two-fold dilution series starting at 2 ng/ml is suggested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Supplied as a lyopholized powder from PBS, pH 7.4, 50ug BSA.

Reconstitute with 250uL sterile PBS.

Store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01137 NCBI: NM_000660 NP_000651.3