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Anti-TDP-43 / TARDBP Antibody (clone 3H8) LS-C204584

Ordering

Wt. Vol. Conc. Price
- 100 µl - $285

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TDP-43 / TARDBP Antibody LS-C204584 Images

[click image for a larger image and more details]
Immunohistochemistry
TDP-43 / TARDBP antibody was used to stain a section of formalin fixed adult rat brain,...
Western Blot
Blots of TDP-43 / TARDBP antibody on crude extract of mouse brain nuclear fraction (left lane)...

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Please Note - This antibody was previously listed as catalog number LS-C180210. Please update your records to reflect the changed catalog number.
Mouse Monoclonal [clone 3H8] (IgG1) to Human TDP-43 / TARDBP
Human, Mouse, Rat, Bovine, Pig
IHC, Immunofluorescence, Western blot
Unconjugated

Details

Human TDP-43 / TARDBP
Mouse
Human, Mouse, Rat, Bovine, Pig (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [3H8]
Affinity purified

Applications

IHC (1:1000), Immunofluorescence (1:1000), Western blot (1:5000)

Specificity and Use

TDP-43 / TARDBP antibody was raised against full length recombinant human TDP43 expressed in and purified from E. coli.
Clone 3H8 is known to react with TDP43 from human, cow, pig, mouse, rat and other mammals. Since the TDP43 protein sequence is highly conserved across species barriers, it is likely that the antibody is effective on many other species also.
Try at dilutions of 1:1000 and higher for immunofluorescence, 1:5000 and lower for western blotting.

Packaging

Liquid
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

About TDP-43 / TARDBP

Q13148 NM_007375 NP_031401.1

TARDBP Antibody, ALS10 Antibody, TAR DNA-binding protein-43 Antibody, TDP-43 Antibody, TAR DNA binding protein Antibody, TAR DNA-binding protein 43 Antibody, TDP43 Antibody


Immunohistochemistry

Immunohistochemistry
TDP-43 / TARDBP antibody was used to stain a section of formalin fixed adult rat brain, specifically the hippocampus. Hippocampal neuron nuclei are stained strongly. Chicken antibody to GFAP CPCA-GFAP (green) shows the processes of astrocytic glial cells. Nuclei of all cells are revealed with DAPI DNA stain (blue). The TARDP antibody stains neuronal nuclei strongly and the nuclei of some non-neuronal cells much more weakly. Neuronal nuclei therefore look crimson, since they are both red due to the content of TDP43 and blue due to their content of DNA, stained blue with DAPI.

Western Blot

Western Blot
Blots of TDP-43 / TARDBP antibody on crude extract of mouse brain nuclear fraction (left lane) and cytoplasmic fraction (right lane) blotted with monoclonal antibody TDP-43 / TARDBP antibody. There is a strong clear band in the nuclear preparation running at 43kDa, and a much more minor band in the cytoplasmic fraction.

Requested From: United States
Date Requested: 9/2/2014

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