Human, Mouse, Rat, Bovine, Guinea pig, Horse, Zebrafish
Human SPON2 / MINDIN
Human, Mouse, Rat, Bovine, Guinea pig, Horse, Zebrafish (tested or 100% immunogen sequence identity)
Xenopus (at least 90% immunogen sequence identity)
Western blot (0.2 - 1 µg/ml)
Specificity and Use
SPON2 / MINDIN antibody was raised against synthetic peptide from N-terminus of human SPON2 (NP_036577.1, Q9BUD6). Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Horse, Guinea pig, Zebrafish (100%); Chicken, Xenopus (92%).
Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
Lyophilized from PBS with 2% sucrose
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.