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Anti-SMAD3 Antibody (phospho-Ser423/Ser425) IHC-plus™ LS-B64

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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SMAD3 Antibody LS-B64 Images

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Immunohistochemistry
Anti-SMAD3 antibody IHC of human skin.

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Rabbit Polyclonal to Human SMAD3
Human, Mouse, Rat, Bovine, Pig, Chicken, Xenopus, Zebrafish
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human SMAD3
Rabbit
Human, Mouse, Rat, Bovine, Pig, Chicken, Xenopus, Zebrafish (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (2.5 µg/ml), Immunofluorescence, Western blot (1:2000 - 1:20000), Flow Cytometry, ELISA (1:75000 - 1:300000)

Specificity and Use

SMAD3 antibody was raised against synthetic peptide from human Smad3.
pSer423/Ser425
Amino acids 417-425 of human SMAD3 protein.
Immunohistochemistry: LS-B64 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B64 was determined to be 2.5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
For research use only.

About SMAD3

P84022 NM_005902 NP_005893.1

SMAD3 Antibody, HSPC193 Antibody, HMAD-3 Antibody, HsT17436 Antibody, LDS1C Antibody, LDS3 Antibody, Mad homolog JV15-2 Antibody, MAD3 Antibody, MADH3 Antibody, Mothers against DPP homolog 3 Antibody, JV15-2 Antibody, SMA- and MAD-related protein 3 Antibody, SMAD family member 3 Antibody, MAD homolog 3 Antibody, HSMAD3 Antibody, Mad protein homolog Antibody, SMAD 3 Antibody

SMAD3, a transcriptional regulator, is activated by TGF-beta and Activin 1 receptor kinase. SMAD3 recognizes an 8 bp palindromic sequence GTCTAGAC. Upon phosphorylation at two extreme C-terminal serines, SMAD3 is translocated into the nucleus where is forms complexes other SMAD proteins, depending on the activation pathway, and regulates the transcription of target genes.

Immunohistochemistry

Immunohistochemistry
Anti-SMAD3 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B64 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 10/22/2014

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