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Catalog Number	Size	Price
LS-B9109-200	200 µl	$460

SMAD2 Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

SMAD2 Antibody - Fluorescent confocal image of SY5Y cells stained with SMAD2 (T220) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated SMAD2 (T220) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the SMAD2 mainly to the nucleus.

SMAD2 Antibody - Western blot of SMAD2 Antibody (T220) in NCI-H460 cell line lysates (35 ug/lane). SMAD2 (arrow) was detected using the purified antibody.

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IHC‑plus™ Polyclonal Rabbit anti‑Human SMAD2 Antibody (Thr220, IHC, IF, WB) LS‑B9109

Name: IHC-plus™ SMAD2 Polyclonal Antibody Rabbit anti-Human IF,IHC,WB | LSBio
Brand: Lifespan BioSciences
SKU: LS-B9109-200
Price: 460.0000 USD
Availability: InStock
Rating: 4 (1 reviews)

IHC Validated

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Note: This antibody replaces LS-C100547

Antibody:

SMAD2 Rabbit anti-Human Polyclonal (Thr220) Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

$460

LS-B9109-200

Toll Free North America

206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

SMAD2 Rabbit anti-Human Polyclonal (Thr220) Antibody

Application:

IHC, IHC-P, IF, WB

Reactivity:

Human

Format:

Unconjugated, Unmodified

Specifications

Description

SMAD2 antibody LS-B9109 is an unconjugated rabbit polyclonal antibody to human SMAD2 (Thr220). Validated for IF, IHC and WB. Tested on 20 paraffin-embedded human tissues.

Target

Human SMAD2

Synonyms

See All SMAD2 Antibodies

Host

Rabbit

Reactivity

Human (tested or 100% immunogen sequence identity)

Predicted

Mouse, Rat, Zebrafish, Cow (at least 90% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Protein A purified

Modifications

Unmodified

Epitope

Thr220

Specificity

This SMAD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 201-230 amino acids from human SMAD2.

Applications

IHC
IHC - Paraffin (10 µg/ml)
Immunofluorescence (1:200)
Western blot (1:1000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS with 0.09% (w/v) sodium azide

Storage

Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About SMAD2

Uniprot: Q15796 NCBI: NM_005901 NP_005892.1