Human SLC2A2 / GLUT2

Mouse

Human (tested or 100% immunogen sequence identity)

IgG2a Monoclonal

199017

Unconjugated

Protein G purified

Unmodified

Recombinant protein corresponding to Met1-Val524 from human GLUT 2 expressed in NS0 cells.

Recognizes human GLUT 2 (Glucose Transporter, Insulin Regulatable) expression on hGlut2-transfected NS0 cells but not on control transfectants. Detects Glut2 on the surface of the human intestinal cell line Caco2 in flow cytometry and on fixed cells in immunocytochemistry tests. Based on flow cytometric tests on transfected cells, this antibody has no cross-reactivity with hGlut1 or hGlut3.

• IHC
• IHC - Paraffin (10 µg/ml)
• ICC (10 µg/ml)
• Flow Cytometry

Flow Cytometry: 0.25ug labels 1x10e6 HepG2 human; Immunohistochemistry: LS-B2421 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2421 was determined to be 10 ug/ml.

PBS, pH 7.4, 5% Trehalose

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P11168 NCBI: NM_000340 NP_000331.1