Human Antithrombin-III

Goat

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity absorbed

Unmodified

Human Antithrombin III.

Human SERPINC1 / Antithrombin-III. The reactivity of the antiserum is restricted to antithrombin III. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified antithrombin III. No reaction is obtained with any other plasma protein including the main proteinase inhibitors known to have structural homology with antithrombin III. Protein complexes containing antithrombin III may also react. Cross-reactivity The antiserum does not cross react with any other component of human plasma. Inter-species cross- reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, however in double radial immunodiffusion a reaction has been found with Rhesus monkey.

• IHC
• Immunodiffusion

The anodic mobility enables quantitative determination of antithrombin III using the standard EID technique (Laurell). Immunoelectrophoresis and radial immunodiffusion are used to identify antithrombin III and enzyme inhibitor complexes. Double radial immunodiffusion (Mancini) is also used for quantitative assays. If applied in more sensitive test procedures, in Immunohistochemistry, or solid phase immunoassays, appropriate specificity controls should always be included. Plasma samples and all assay components must contain EDTA to stabilize the proteins.

Lyophilized antiserum

Use 1 ml of sterile Distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P01008 NCBI: NM_000488 NP_000479.1