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Anti-SERPINA1 / Alpha 1 Antitrypsin Antibody (clone 11G2) IHC-plus™ LS-B10262

Ordering

Wt. Vol. Conc. Price
- 50 µl 1.2 mg/ml $395

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SERPINA1 / Alpha 1 Antitrypsin Antibody LS-B10262 Images

[click image for a larger image and more details]
Immunohistochemistry
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-SERPINA1 monoclonal...
Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1...
Immunoprecipitation
Immunoprecipitation(IP) of SERPINA1 by using monoclonal anti-SERPINA1 antibodies (Negative...

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Mouse Monoclonal [clone 11G2] (IgG1) to Human SERPINA1 / Alpha 1 Antitrypsin
Human, Monkey, Dog
IHC - Paraffin, Western blot, Immunoprecipitation
Unconjugated

Details

Human SERPINA1 / Alpha 1 Antitrypsin
Mouse
Human, Monkey, Dog (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [11G2]
Protein A/G purified

Applications

IHC - Paraffin (10 µg/ml), Western blot (1:1000 - 1:2000), Immunoprecipitation

Specificity and Use

SERPINA1 / Alpha 1 Antitrypsin antibody was raised against protein expressed in 293T cell transfected with human SERPINA1 expression vector.
Human SERPINA1 / Alpha 1 Antitrypsin
Concentration: 0.5-1.0 mg/ml (Lot dependent)

Packaging

PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About SERPINA1 / Alpha 1 Antitrypsin

P01009 K02212 AAB59495.1

SERPINA1 Antibody, A1A Antibody, A1AT Antibody, AAT Antibody, Alpha 1 Antitrypsin Antibody, Alpha-1 protease inhibitor Antibody, Alpha-1-antiproteinase Antibody, Alpha-1-antitrypsin Antibody, Alpha1AT Antibody, PRO2275 Antibody, Alpha-1-antitrypsin null Antibody, PI1 Antibody, Serpin A1 Antibody


Immunohistochemistry

Immunohistochemistry
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western Blot

Western Blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-SERPINA1 monoclonal antibody.

Western Blot

Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINA1.

Immunoprecipitation

Immunoprecipitation
Immunoprecipitation(IP) of SERPINA1 by using monoclonal anti-SERPINA1 antibodies (Negative control: IP without adding anti-SERPINA1 antibody.). For each experiment, 500ul of DDK tagged SERPINA1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-SERPINA1 antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Requested From: United States
Date Requested: 10/20/2014

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