Human RELA / NFKB p65

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Delipidated and defibrinated

Unmodified

NFkB p65 (Rel A) peptide corresponding to an internal region near phospho Serine 276 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

pSer276

This phospho specific polyclonal antibody is specific for phosphorylated pS276 human p65. Reactivity with non-phosphorylated pS267 is minimal. Cross reactivity with pS276 phosphorylated p65 from mouse, rat or other species has not been determined.

• IHC
• Western blot (1:1000)
• Immunoprecipitation
• ELISA (1:5000 - 1:25000)

This phospho specific polyclonal antibody reacts human pS276 p65 and shows minimal reactivity by western blot with non-phosphorylated p65 and minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. For immunoblotting a 1:1000 dilution is recommended. A 65 kD band corresponding to human p65 is detected. HeLa cells or TNF inducible KBM-5 cells can be used as a positive control.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q04206 NCBI: NM_021975 NP_068810.3