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Catalog Number Size Price
LS-C153943-500 500 µg (1 mg/ml) $480 
Rat IgG Antibody - ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH

Polyclonal Goat Anti‑Rat IgG Secondary Antibody (Atto 425, IF, WB) LS‑C153943

Polyclonal Goat Anti‑Rat IgG Secondary Antibody (Atto 425, IF, WB) LS‑C153943

Antibody:
Rat IgG Goat anti-Rat Polyclonal (Atto 425) Antibody
Application:
IF, WB, FLISA
Reactivity:
Rat
Format:
Atto 425, Unmodified
Price
Catalog Number
$480
LS-C153943-500
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Rat IgG Goat anti-Rat Polyclonal (Atto 425) Antibody
Application:
IF, WB, FLISA
Reactivity:
Rat
Format:
Atto 425, Unmodified

Specifications

Description
Rat IgG antibody LS-C153943 is an Atto 425-conjugated goat polyclonal antibody. Validated for FLISA, IF and WB.
Host
Goat
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Atto 425
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Rat IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins. This antibody will react with heavy chains of rat IgG and with light chains of most rat immunoglobulins.
Applications
  • Immunofluorescence (1:5000)
  • Western blot (1:10000)
  • Fluorophore-Linked Immunosorbent Assay (1:20000)
Usage
The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 500 µL of deionized water.
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/16/2024