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Anti-RAG2 / RAG-2 Antibody (aa271-527) LS-C186092

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.37 mg/ml $335

LSBio (Direct) LSBio (Direct)
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866-206-6909
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RAG2 / RAG-2 Antibody LS-C186092 Images

[click image for a larger image and more details]
Immunohistochemistry
IHC of paraffin-embedded DLD1 Xenograft using RAG2 antibody at 1:100 dilution.
ICC
Immunofluorescence of paraformaldehyde-fixed HeLa using RAG2 antibody at 1:200 dilution.
Western Blot
Sample (30 ug of whole cell lysate).
 

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Rabbit Polyclonal (IgG) to Human RAG2 / RAG-2
Human
IHC - Paraffin, ICC, Western blot
Unconjugated

Details

Human RAG2 / RAG-2
Rabbit
Human (tested or 100% immunogen sequence identity)
Bovine, Pig, Rabbit (at least 90% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (1:100 - 1:1000), ICC (1:100 - 1:1000), Western blot (1:500 - 1:3000)

Specificity and Use

RAG2 / RAG-2 antibody was raised against recombinant fragment corresponding to a region within amino acids 271 and 527 of RAG2 (SwissProt P55895). Percent identity by BLAST analysis: Human (100%); Rabbit, Bovine (91%); Pig (90%); Rat (86%); Mouse (85%).
aa271-527
Human RAG2 / RAG-2
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About RAG2 / RAG-2

P55895 M94633 AAH22397.1

RAG2 Antibody, RAG-2 Antibody


Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded DLD1 Xenograft using RAG2 antibody at 1:100 dilution.

ICC

ICC
Immunofluorescence of paraformaldehyde-fixed HeLa using RAG2 antibody at 1:200 dilution.

Western Blot

Western Blot
Sample (30 ug of whole cell lysate). A: Molt-4 . 7.5% SDS PAGE. RAG2 / RAG-2 antibody diluted at 1:1000.
 

Requested From: United States
Date Requested: 10/20/2014

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