Human (tested or 100% immunogen sequence identity)
Bovine, Pig, Rabbit (at least 90% immunogen sequence identity)
IHC - Paraffin (1:100 - 1:1000), ICC (1:100 - 1:1000), Western blot (1:500 - 1:3000)
Specificity and Use
RAG2 / RAG-2 antibody was raised against recombinant fragment corresponding to a region within amino acids 271 and 527 of RAG2 (SwissProt P55895). Percent identity by BLAST analysis: Human (100%); Rabbit, Bovine (91%); Pig (90%); Rat (86%); Mouse (85%).
Human RAG2 / RAG-2
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.