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Antibodies > Rabbit IgG > LS-C60865
 
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Catalog Number Size Price
LS-C60865-1 1 mg (1 mg/ml) $417 
Rabbit IgG Antibody - HRP-conjugated Goat-Anti-Rabbit secondary antibody-Western blot. HRP-conjugated Goat-Anti-Rabbit (Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins)) secondary antibody was used at 1:40000 in MB-070 blocking buffer to detect a rabbit primary antibody by Western Blot. Anti-p27 antibody (200-401-C30, 1:1000 RT 30 minutes) showed detection of 0.1 ug of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kD). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 micron nitrocellulose and blocked with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4?. Blot was imaged on the VersaDoc MP 4000 imaging system (Bio-Rad). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Rabbit IgG Antibody - secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein (011-0102, 250ng starting total load) co incubated with HRP conjugated Goat anti Rabbit IgG (Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins)) and Dylight 649 conjugated goat anti Rabbit (611-143-122) 1:20K in MB-070. Blot was dried and imaged (A) on Bio-Rad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager, This image was taken for the unconjugated form of this product. Other forms have not been tested.
Rabbit IgG Antibody - HRP-conjugated Goat-Anti-Rabbit secondary antibody-Western blot. HRP-conjugated Goat-Anti-Rabbit (Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins)) secondary antibody was used at 1:40000 in MB-070 blocking buffer to detect a rabbit primary antibody by Western Blot. Anti-p27 antibody (200-401-C30, 1:1000 RT 30 minutes) showed detection of 0.1 ug of recombinant p27 protein. Lane 1: Molecular weight markers. Lane 2: MBP-p27 fusion protein (arrow; expected MW: 73.3 kD). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 micron nitrocellulose and blocked with 1% BSA-TTBS (p/n MB-013, diluted to 1X) overnight at 4?. Blot was imaged on the VersaDoc MP 4000 imaging system (Bio-Rad). This image was taken for the unconjugated form of this product. Other forms have not been tested.
Rabbit IgG Antibody - secondary antibodies detect rabbit primary antibodies in a variety of platforms. Shown here is a serial 1:1 dilution of control rabbit IgG protein (011-0102, 250ng starting total load) co incubated with HRP conjugated Goat anti Rabbit IgG (Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins)) and Dylight 649 conjugated goat anti Rabbit (611-143-122) 1:20K in MB-070. Blot was dried and imaged (A) on Bio-Rad Versa Doc (30 sec, DyLight649), (B) LiCor Odyssey Reader (700 nm), (C ) Rewetted incubated with Femtomax 110 reimaged using BioVersaDoc (for 60 sec), (D) Incubated with TMB substrate TMBM for 5 minutes and scanned, and (E) Rewetted for Chemiluminescence and imaged for 90 sec on the BioRad VersaDoc Imager, This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Polyclonal Goat Anti‑Rabbit IgG Secondary Antibody (HRP, IHC, WB) LS‑C60865

Polyclonal Goat Anti‑Rabbit IgG Secondary Antibody (HRP, IHC, WB) LS‑C60865

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Antibody:
Rabbit IgG Goat anti-Rabbit Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA, DB
Reactivity:
Rabbit
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$417
LS-C60865-1
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Rabbit IgG Goat anti-Rabbit Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA, DB
Reactivity:
Rabbit
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
Rabbit IgG antibody LS-C60865 is an HRP-conjugated goat polyclonal antibody. Validated for DB, ELISA, IHC and WB.
Target
Rabbit IgG
Host
Goat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Rabbit IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:2000 - 1:10000)
  • ELISA (1:20000 - 1:100000)
  • Dot Blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Store at 4°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Featured Products

Rabbit IgG Antibody - Western Blot of Goat anti-Rabbit IgG Pre-Absorbed Phycoerythrin Conjugated Antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT.
Goat Anti‑Rabbit IgG Secondary Antibody (RPE) LS‑C60868
Species: Rabbit
Applications: Immunofluorescence, Western blot, Flow Cytometry
Rabbit IgG Antibody - RABBIT IgG (H&L) Antibody Peroxidase Conjugated Western Blot. Western blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody Lane 1: HeLa Whole Cell Lysate Lane 2: NIH/3T3 Whole Cell Lysate Load: 10 ug per lane Primary antibody: Beta Actin Antibody at 1:1000 for overnight at 4C Secondary antibody: Peroxidase Conjugated Goat Anti-Rabbit Antibody at 1:40000 for 30 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 42 kD, 42 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Goat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C60884
Species: Rabbit
Applications: IHC, Western blot, ELISA
Rabbit IgG Antibody - Western Blot of Fluorescein conjugated Goat anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT.
Goat Anti‑Rabbit IgG Secondary Antibody (FITC) LS‑C60881
Species: Rabbit
Applications: Immunofluorescence, Western blot, Flow Cytometry
Rabbit IgG Antibody - HRP conjugated Anti-Rabbit IgG polyclonal antibody-Western blot. HRP conjugated anti-rabbit antibody was used to detect anti-Beta Actin antibody (LS-B323 lot 26928). HeLa (Lane 1) and NIH 3T3 (Lane 2) Whole cell lysates were run on a 4-20% gel, transferred to nitrocellulose under standard conditions, and incubated with anti-beta actin at a dilution of 1:2000 (ON 4C). For secondary antibody detection, blot was incubated for 1 hr RT simultaneously with: 1. ATTO 647N conjugated anti-rabbit antibody (p/n lot 26426C, 1:10000 in MB-070, Shown on Left and 2. HRP conjugated anti-rabbit IgG (Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Human Serum Proteins) lot 19247, 1:10000 in MB-070, shown on right) Blot was dried, imaged at a wavelength of 700 nm on a LiCor Odyssey reader, rewetted in TBS and imaged after 2 min with a 30 sec exposure time using Femtomax-110 super sensitive Chemiluminescent substrate using the Bio-Rad Versa Doc Imaging System. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Goat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C60887
Species: Rabbit
Applications: IHC, Western blot, ELISA, Dot Blot, Electron Microscopy
Product
Goat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C56309
Species: Rabbit
Applications: IHC - Frozen, Western blot, ELISA
Rabbit IgG Antibody - Western blot of Peroxidase conjugated Rat Anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rat secondary antibody at 1:1,000 for 60 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.
Rat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C60921
Species: Rabbit
Applications: IHC, Western blot, ELISA
See all Rabbit IgG Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/22/2024
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