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Antibodies > Rabbit IgG > LS-C61475
 
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Catalog Number Size Price
LS-C61475-500 500 µg (1 mg/ml) $352 
Rabbit IgG Antibody - Western blot of Fluorescein conjugated Goat F(ab’)2 Anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.

Polyclonal Goat Anti‑Rabbit IgG Secondary Antibody (FITC, IF, WB) LS‑C61475

Polyclonal Goat Anti‑Rabbit IgG Secondary Antibody (FITC, IF, WB) LS‑C61475

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Antibody:
Rabbit IgG Goat anti-Rabbit Polyclonal (FITC) Antibody
Application:
IF, WB, Flo, FLISA
Reactivity:
Rabbit
Format:
FITC, Unmodified
Price
Catalog Number
$352
LS-C61475-500
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Rabbit IgG Goat anti-Rabbit Polyclonal (FITC) Antibody
Application:
IF, WB, Flo, FLISA
Reactivity:
Rabbit
Format:
FITC, Unmodified

Specifications

Description
Rabbit IgG antibody LS-C61475 is an FITC-conjugated goat polyclonal antibody. Validated for FLISA, Flow, IF and WB.
Target
Rabbit IgG
Host
Goat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG F(ab')2 Polyclonal
Conjugations
FITC
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Rabbit IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against anti-Pepsin, anti-Goat IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
Applications
  • Immunofluorescence (1:1000 - 1:5000)
  • Western blot
  • Flow Cytometry (1:500 - 1:2500)
  • Fluorophore-Linked Immunosorbent Assay (1:10000 - 1:50000)
Usage
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 500 µL of deionized water.
Storage
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted. Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Featured Products

Rabbit IgG Antibody - Western Blot of Goat anti-Rabbit IgG Pre-Absorbed Phycoerythrin Conjugated Antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT.
Goat Anti‑Rabbit IgG Secondary Antibody (RPE) LS‑C60868
Species: Rabbit
Applications: Immunofluorescence, Western blot, Flow Cytometry
Rabbit IgG Antibody - RABBIT IgG (H&L) Antibody Peroxidase Conjugated Western Blot. Western blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody Lane 1: HeLa Whole Cell Lysate Lane 2: NIH/3T3 Whole Cell Lysate Load: 10 ug per lane Primary antibody: Beta Actin Antibody at 1:1000 for overnight at 4C Secondary antibody: Peroxidase Conjugated Goat Anti-Rabbit Antibody at 1:40000 for 30 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 42 kD, 42 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Goat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C60884
Species: Rabbit
Applications: IHC, Western blot, ELISA
Rabbit IgG Antibody - Western Blot of Fluorescein conjugated Goat anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT.
Goat Anti‑Rabbit IgG Secondary Antibody (FITC) LS‑C60881
Species: Rabbit
Applications: Immunofluorescence, Western blot, Flow Cytometry
Rabbit IgG Antibody - HRP conjugated Anti-Rabbit IgG polyclonal antibody-Western blot. HRP conjugated anti-rabbit antibody was used to detect anti-Beta Actin antibody (LS-B323 lot 26928). HeLa (Lane 1) and NIH 3T3 (Lane 2) Whole cell lysates were run on a 4-20% gel, transferred to nitrocellulose under standard conditions, and incubated with anti-beta actin at a dilution of 1:2000 (ON 4C). For secondary antibody detection, blot was incubated for 1 hr RT simultaneously with: 1. ATTO 647N conjugated anti-rabbit antibody (p/n lot 26426C, 1:10000 in MB-070, Shown on Left and 2. HRP conjugated anti-rabbit IgG (Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Human Serum Proteins) lot 19247, 1:10000 in MB-070, shown on right) Blot was dried, imaged at a wavelength of 700 nm on a LiCor Odyssey reader, rewetted in TBS and imaged after 2 min with a 30 sec exposure time using Femtomax-110 super sensitive Chemiluminescent substrate using the Bio-Rad Versa Doc Imaging System. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Goat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C60887
Species: Rabbit
Applications: IHC, Western blot, ELISA, Dot Blot, Electron Microscopy
Product
Goat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C56309
Species: Rabbit
Applications: IHC - Frozen, Western blot, ELISA
Rabbit IgG Antibody - Western blot of Peroxidase conjugated Rat Anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rat secondary antibody at 1:1,000 for 60 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.
Rat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C60921
Species: Rabbit
Applications: IHC, Western blot, ELISA
See all Rabbit IgG Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
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