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Antibodies > Rabbit IgG > LS-C60912
 
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Catalog Number Size Price
LS-C60912-1000 1000 µg (1 mg/ml) $327 
Rabbit IgG Antibody - Western Blot of Anti-Rabbit IgG (H&L) (MOUSE) Antibody (Min X Hu, Gt, Ms Serum Proteins) Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule Lane 2: Rabbit IgG F(ab) Fragment Lane 3: Rabbit IgG F(c) Fragment Lane 4: Rabbit IgM Whole Molecule Lane 5: Normal Rabbit Serum All samples were reduced. Load: 50 ng per lane.

Polyclonal Mouse Anti‑Rabbit IgG Secondary Antibody (IHC, WB) LS‑C60912

Polyclonal Mouse Anti‑Rabbit IgG Secondary Antibody (IHC, WB) LS‑C60912

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Antibody:
Rabbit IgG Mouse anti-Rabbit Polyclonal Antibody
Application:
IHC, WB, ELISA, IE
Reactivity:
Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$327
LS-C60912-1000
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Rabbit IgG Mouse anti-Rabbit Polyclonal Antibody
Application:
IHC, WB, ELISA, IE
Reactivity:
Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
Rabbit IgG antibody LS-C60912 is an unconjugated mouse polyclonal antibody. Validated for ELISA, IE, IHC and WB.
Target
Rabbit IgG
Host
Mouse
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in mouse.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Goat, Human or Mouse Serum Proteins.
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:3000 - 1:30000)
  • ELISA (1:65000)
  • Immunoelectrophoresis
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

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Featured Products

Rabbit IgG Antibody - Western Blot of Goat anti-Rabbit IgG Pre-Absorbed Phycoerythrin Conjugated Antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT.
Goat Anti‑Rabbit IgG Secondary Antibody (RPE) LS‑C60868
Species: Rabbit
Applications: Immunofluorescence, Western blot, Flow Cytometry
Rabbit IgG Antibody - RABBIT IgG (H&L) Antibody Peroxidase Conjugated Western Blot. Western blot of Peroxidase Conjugated Goat Anti-Rabbit Antibody Lane 1: HeLa Whole Cell Lysate Lane 2: NIH/3T3 Whole Cell Lysate Load: 10 ug per lane Primary antibody: Beta Actin Antibody at 1:1000 for overnight at 4C Secondary antibody: Peroxidase Conjugated Goat Anti-Rabbit Antibody at 1:40000 for 30 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 42 kD, 42 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Goat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C60884
Species: Rabbit
Applications: IHC, Western blot, ELISA
Rabbit IgG Antibody - Western Blot of Fluorescein conjugated Goat anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein goat secondary antibody at 1:1,000 for 60 min at RT.
Goat Anti‑Rabbit IgG Secondary Antibody (FITC) LS‑C60881
Species: Rabbit
Applications: Immunofluorescence, Western blot, Flow Cytometry
Rabbit IgG Antibody - HRP conjugated Anti-Rabbit IgG polyclonal antibody-Western blot. HRP conjugated anti-rabbit antibody was used to detect anti-Beta Actin antibody (LS-B323 lot 26928). HeLa (Lane 1) and NIH 3T3 (Lane 2) Whole cell lysates were run on a 4-20% gel, transferred to nitrocellulose under standard conditions, and incubated with anti-beta actin at a dilution of 1:2000 (ON 4C). For secondary antibody detection, blot was incubated for 1 hr RT simultaneously with: 1. ATTO 647N conjugated anti-rabbit antibody (p/n lot 26426C, 1:10000 in MB-070, Shown on Left and 2. HRP conjugated anti-rabbit IgG (Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated (Min X Human Serum Proteins) lot 19247, 1:10000 in MB-070, shown on right) Blot was dried, imaged at a wavelength of 700 nm on a LiCor Odyssey reader, rewetted in TBS and imaged after 2 min with a 30 sec exposure time using Femtomax-110 super sensitive Chemiluminescent substrate using the Bio-Rad Versa Doc Imaging System. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Goat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C60887
Species: Rabbit
Applications: IHC, Western blot, ELISA, Dot Blot, Electron Microscopy
Product
Goat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C56309
Species: Rabbit
Applications: IHC - Frozen, Western blot, ELISA
Rabbit IgG Antibody - Western blot of Peroxidase conjugated Rat Anti-Rabbit IgG Pre-Adsorbed secondary antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase rat secondary antibody at 1:1,000 for 60 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG. Other band(s): None.
Rat Anti‑Rabbit IgG Secondary Antibody (HRP) LS‑C60921
Species: Rabbit
Applications: IHC, Western blot, ELISA
See all Rabbit IgG Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
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