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Anti-PTGS2 / COX2 / COX-2 Antibody (clone COX 229) IHC-plus™ LS-B3296

Ordering

Wt. Vol. Conc. Price
- 250 µl 0.18 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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PTGS2 / COX2 / COX-2 Antibody LS-B3296 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-COX-2 antibody IHC of human skeletal muscle.
Immunohistochemistry
Anti-COX-2 antibody IHC of human small intestine, ganglion cells.

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Mouse Monoclonal [clone COX 229] (IgG1,k) to Human PTGS2 / COX2 / COX-2
Human
IHC - Paraffin, IHC - Frozen
Unconjugated

Details

Human PTGS2 / COX2 / COX-2
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [COX 229]
Purified

Applications

IHC - Paraffin (10 µg/ml), IHC - Frozen

Specificity and Use

PTGS2 / COX2 / COX-2 antibody was raised against synthetic peptide from human COX-2 Expected Staining Pattern: Cytoplasm.
Recognizes human COX-2.
Immunohistochemistry: LS-B3296 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3296 was determined to be 10 ug/ml. Positive control: Colon carcinoma.

Packaging

PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About PTGS2 / COX2 / COX-2

P35354 NM_000963 NP_000954.1

PTGS2 Antibody, COX-2 Antibody, Cyclooxygenase-2 Antibody, COX2 Antibody, Cyclooxygenase 2b Antibody, HCox-2 Antibody, GRIPGHS Antibody, PGH synthase 2 Antibody, PGHS-2 Antibody, PHS II Antibody, PHS-2 Antibody, Prostaglandin H2 synthase 2 Antibody, PGG/HS Antibody, Prostaglandin G/H synthase 2 Antibody

Prostaglandin-endoperoxide synthase (fatty acid cyclooxygenase; PGH synthase; Cox-2) is a key enzyme in prostaglandin biosynthesis. The first rate-limiting step in the conversion of arachidonic acid to prostaglandins is catalyzed by Cox-1 and Cox-2. The cyclooxygenase activity of Cox-2 is inhibited by nonsteroidal antiinflammatory drugs such as aspirin and endomethacin.

Immunohistochemistry

Immunohistochemistry
Anti-COX-2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3296 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-COX-2 antibody IHC of human small intestine, ganglion cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3296 concentration 10 ug/ml.

Requested From: United States
Date Requested: 10/22/2014

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