Human POU5F1 / OCT4

Rabbit

Human, Rodent (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated. Also available conjugated with APC, Biotin, FITC, HRP, PE.

Immunoaffinity purified

Unmodified

Synthetic peptide (aa 282-297) of Oct4.

aa282-297

Recognizes Oct4 (POU5F1). MW: 38,.6kD. Species cross-reactivity: Human and rodent.

• IHC (1:200 - 1:1000)
• ICC (1:200 - 1:1000)
• Western blot (1:200 - 1:1000)

Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Western Blot: 1:200-1:1000 using ECL. Immunohistochemistry: 1:200-1:1000. Immunocytochemistry: 1:200-1:1000.Immunohistochemistry protocol:1. Dissect tissues and freeze on dry ice.2. Cut on a cryostat 10um slices.3. Dry slides at RT for 30-90 min4. Fix with cold Acetone: Methanol (50:50) 2 min. Either process further or store at -20ºC. Alternative is fixation with 4% PFA and treatment with 0.05% trypsin solution (incubation 10 min. at 37ºC, followed by 3 washes with PBS)5. Let air dry.6. PBS 5 min7. Incubate in 50mM ammonium chloride 30 min.8. PBS 5 min9. Blocking serum 30-45 min10. Primary antibody (LS-C9379) 90 min11. Wash 3X for 5 min12. Secondary fluorescent conjugated antibody 30 min13. Wash 3X for 5 min.14. Mount.

PBS, pH 7.4, No preservatives added

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q01860 NCBI: NM_002701 NP_002692.2