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Anti-POLR3A Antibody (aa182-482) LS-C185773

Ordering

Wt. Vol. Conc. Price
- 100 µl 0.89 mg/ml $335

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866-206-6909
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POLR3A Antibody LS-C185773 Images

[click image for a larger image and more details]
ICC
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using POLR3A...
Western Blot
Sample (30 ug of whole cell lysate). A: HCT116. 5% SDS PAGE. POLR3A antibody diluted at 1:500.

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Rabbit Polyclonal (IgG) to Human POLR3A
Human
ICC, Western blot
Unconjugated

Details

Human POLR3A
Rabbit
Human (tested or 100% immunogen sequence identity)
Mouse, Bovine, Chicken, Xenopus (at least 90% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

ICC (1:100 - 1:1000), Western blot (1:500 - 1:3000)

Specificity and Use

POLR3A antibody was raised against recombinant fragment corresponding to a region within amino acids 182 and 482 of POLR3A (SwissProt O14802). Percent identity by BLAST analysis: Human (100%); Bovine (98%); Mouse, Chicken (97%); Xenopus (94%).
aa182-482
Human POLR3A

Packaging

0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About POLR3A

O14802 NM_007055 NP_008986.2

POLR3A Antibody, ADDH Antibody, HRPC155 Antibody, RNA polymerase III subunit C1 Antibody, HLD7 Antibody, RPC1 Antibody, RPC155 Antibody


ICC

ICC
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using POLR3A antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Western Blot

Western Blot
Sample (30 ug of whole cell lysate). A: HCT116. 5% SDS PAGE. POLR3A antibody diluted at 1:500.

Requested From: United States
Date Requested: 10/31/2014

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