LSBio The Immunohistochemistry Antibody Company
  • Home
  • Products
    • Primary Antibodies
    • Secondary Antibodies
    • IHC-plus Antibodies
    • Isotype Control Antibodies
  • Services
    • Immunohistochemistry Studies
    • Tissue Cross-Reactivity Screening
  • Distributors
  • Resources
    • About our Tissue Bank
    • Customer Support
    • How to Buy
    • IHC-plus Protocol
    • LSBio Media
    • Publications
    • Antibody Reports
  • Company
    • About LifeSpan
    • Press Releases
    • Holiday Schedule
    • Contact Us
  • View Cart

Anti-PLK1 / PLK-1 Antibody (phospho-Thr210, clone 2A3) IHC-plus™ LS-B1675

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

PLK1 / PLK-1 Antibody LS-B1675 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-PLK1 antibody IHC of human colon.
Western Blot
Panel A.
Western Blot
 

100% Guaranteed 100% Guaranteed Publications Publications (1)
Mouse Monoclonal [clone 2A3] (IgG1) to Human PLK1 / PLK-1
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human PLK1 / PLK-1
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [2A3]
Affinity purified

Applications

IHC - Paraffin (15 µg/ml), Western blot

Specificity and Use

PLK1 / PLK-1 antibody was raised against synthetic peptide from human PLK1.
pThr210
Modified peptide
Immunohistochemistry: LS-B1675 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1675 was determined to be 15 ug/ml.

Packaging

Phosphate-buffered solution, pH 7.2, 0.09% sodium azide at 0.5 mg/ml. Sourced in TCS.
+4°C, avoid freezing
For research use only.

Publications (1)

1
Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A. Molli PR, Li DQ, Bagheri-Yarmand R, Pakala SB, Katayama H, Sen S, Iyer J, Chernoff J, Tsai MY, Nair SS, Kumar R. The Journal of cell biology. 2010 190:101-14. [PubMed:20603326] [PMC:PMC2911675]

About PLK1 / PLK-1

P53350 NM_005030 NP_005021.2

PLK1 Antibody, Polo like kinase Antibody, Polo-like kinase (Drosophila) Antibody, Polo-like kinase 1 Antibody, STPK13 Antibody, Polo (Drosophia)-like kinase Antibody, PLK Antibody, PLK-1 Antibody

PLK, a Polo type protein kinase, has been shown to function in mitosis. The mRNA is absent or expressed at very low levels during the G1 phase, begins to reaccumulate during the S phase, and reaches maximal levels during the G2/M phase. PLK1 may participate in targeting MPF to the nucleus during prophase and in regulating mitotic spindle function. These functions, in addition to its expression in proliferating cells, suggest that PLK1 has a role in cancer progression.

Immunohistochemistry

Immunohistochemistry
Anti-PLK1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1675 concentration 15 ug/ml.

Western Blot

Western Blot
Panel A. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were immunoprecipitated with the pan-PLK mAb (clone 3F8), resolved by electrophoresis, transferred to nitrocellulose and probed with mAb 2A3 reactive against Thr210-phosphorylated PLK-1. Proteins were visualized using an HRP goat anti-mouse secondary Ab and a chemiluminescence detection system. Panel B. Extracts from untreated Hela cells (Lane 1) or overnight nocodazole-treated Hela cells (Lane 2) were resolved by electrophoresis, transferred to nitrocellulose and probed with mAb 2A3 reactive against Thr210-phosphorylated PLK-1. Proteins were visualized using an HRP goat anti-mouse secondary and a chemiluminescence detection system.

Western Blot

Western Blot
 
 

Requested From: United States
Date Requested: 7/23/2014
Copyright © 2014 LifeSpan BioSciences, Inc. All Rights Reserved