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Anti-PHACTR1 Antibody (aa468-517) IHC-plus™ LS-B4000

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

PHACTR1 Antibody LS-B4000 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-PHACTR1 antibody IHC of human tonsil.
Immunohistochemistry
Anti-PHACTR1 antibody IHC of human uterus.
Immunohistochemistry
Anti-PHACTR1 antibody IHC of human heart.
Western Blot
PHACTR1 antibody LS-B4000 Western blot of Jurkat lysate.

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Rabbit Polyclonal (IgG) to Human PHACTR1
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus
IHC - Paraffin, Western blot
Unconjugated

Details

Human PHACTR1
Rabbit
Human, Monkey, Mouse, Rat, Bovine, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (0.2 - 1 µg/ml)

Specificity and Use

PHACTR1 antibody was raised against synthetic peptide located between aa468-517 of human PHACTR1 (Q9C0D0, NP_112210). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Marmoset, Mouse, Rat, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Zebra finch, Chicken, Xenopus (100%); Turkey (81%).
LS-E20010 - Lyophilized - 100 µg - $145.00
aa468-517
Human PHACTR1
Immunohistochemistry: LS-B4000 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4000 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About PHACTR1

Q9C0D0 NM_030948 NP_112210.1

PHACTR1 Antibody, DJ257A7.2 Antibody, RPEL1 Antibody, RPEL Antibody, RPEL repeat containing 1 Antibody, KIAA1733 Antibody


Immunohistochemistry

Immunohistochemistry
Anti-PHACTR1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4000 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-PHACTR1 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4000 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-PHACTR1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4000 concentration 5 ug/ml.

Western Blot

Western Blot
PHACTR1 antibody LS-B4000 Western blot of Jurkat lysate.

Requested From: United States
Date Requested: 11/1/2014

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