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Anti-PH / PAH Antibody (Internal) IHC-plus™ LS-B9223

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395

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PH / PAH Antibody LS-B9223 Images

[click image for a larger image and more details]
Immunohistochemistry
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western Blot
PAH antibody (0.3 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer).

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Goat Polyclonal to Human PH / PAH
Human, Gorilla, Orangutan, Gibbon, Monkey, Bat, Dog, Horse, Rabbit
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human PH / PAH
Goat
Human, Gorilla, Orangutan, Gibbon, Monkey, Bat, Dog, Horse, Rabbit (tested or 100% immunogen sequence identity)
Mouse, Rat, Bovine, Chicken, Xenopus (at least 90% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (3.75 µg/ml), Western blot (0.1 - 0.3 µg/ml), ELISA (1:32000)

Specificity and Use

PH / PAH antibody was raised against synthetic peptide C-ESRPSRLKKDE from an internal region of human PAH / Phenylalanine Hydroxylase (NP_000268.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Elephant, Panda, Dog, Bat, Horse, Rabbit (100%); Mouse, Rat, Bovine, Turkey, Chicken, Lizard, Xenopus (91%); Hamster, Opossum, Platypus, Drosophila, Nematode (82%).
LS-E27471 - Lyophilized - 100 µg - $145.00
Internal
Human PAH / Phenylalanine Hydroxylase.
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 48kD band observed in Human Liver lysates (calculated MW of 51.9kD according to NP_000268.1). Recommended concentration: 0.3-1 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About PH / PAH

P00439 NM_000277 NP_000268.1

PAH Antibody, Phe-4-monooxygenase Antibody, PKU1 Antibody, PH Antibody, Phenylalanine 4-monooxygenase Antibody, Phe-4- monooxygenase Antibody, Phenylalanine hydroxylase Antibody, Phenylalanine-4-hydroxylase Antibody, PKU Antibody

PAH encodes the enzyme phenylalanine hydroxylase that is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria.

Immunohistochemistry

Immunohistochemistry
Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)

Western Blot

Western Blot
PAH antibody (0.3 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: United States
Date Requested: 10/31/2014

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