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Anti-PDX1 / PDX-1 Antibody (aa198-213) IHC-plus™ LS-B3086

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

PDX1 / PDX-1 Antibody LS-B3086 Images

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Immunohistochemistry
Anti-PDX1 antibody IHC of human pancreas.

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Rabbit Polyclonal (IgG) to Human PDX1 / PDX-1
Human, Chimpanzee, Gorilla, Gibbon, Monkey, Rabbit
IHC - Paraffin, ELISA
Unconjugated

Details

Human PDX1 / PDX-1
Rabbit
Human, Chimpanzee, Gorilla, Gibbon, Monkey, Rabbit (tested or 100% immunogen sequence identity)
Bat (at least 90% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), ELISA

Specificity and Use

PDX1 / PDX-1 antibody was raised against synthetic peptide, aa198-213 RMKWKKEEDKKRGGGT corresponding to human Pdx1 (KLH). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Rabbit (100%); Bat, Opossum (94%); Mouse, Rat, Hamster, Dog (88%); Turkey, Chicken, Xenopus (81%).
aa198-213
Recognizes human Pdx1. Species cross-reactivity: rodent.
Immunohistochemistry: LS-B3086 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3086 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.2, sodium azide, before the addition of glycerol to 40%
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About PDX1 / PDX-1

P52945 NM_000209 NP_000200.1

PDX1 Antibody, Glucose-sensitive factor Antibody, Insulin upstream factor 1 Antibody, IPF1 Antibody, Insulin promoter factor 1 Antibody, IPF-1 Antibody, IUF-1 Antibody, STF-1 Antibody, GSF Antibody, IDX-1 Antibody, Islet/duodenum homeobox-1 Antibody, IUF1 Antibody, MODY4 Antibody, PDX-1 Antibody

Insulin promoter factor 1 is an islet-specific protein that activates transcription of the insulin andsomatostatin genes. It is a key regulator of islet peptide hormone expressionn and also plays an essential role in pancreatic development. Mutations in this gene may be involved in several disorders of the pancreas or in diabetes mellitus.

Immunohistochemistry

Immunohistochemistry
Anti-PDX1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3086 concentration 5 ug/ml.
 

Requested From: United States
Date Requested: 10/20/2014

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