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| Catalog Number | Size | Price |
|---|---|---|
| LS-B2457-50 | 50 µg (0.5 mg/ml) | $485 |
1 of 2
2 of 2
IHC‑plus™ Polyclonal Goat anti‑Human PC / Pyruvate Carboxylase Antibody (aa886‑899, IHC, WB) LS‑B2457
IHC‑plus™ Polyclonal Goat anti‑Human PC / Pyruvate Carboxylase Antibody (aa886‑899, IHC, WB) LS‑B2457
Note: This antibody replaces LS-C55348
Antibody:
PC / Pyruvate Carboxylase Goat anti-Human Polyclonal (aa886-899) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
PC / Pyruvate Carboxylase Goat anti-Human Polyclonal (aa886-899) Antibody
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
Format:
Unconjugated, Unmodified
Specifications
Description
Pyruvate Carboxylase antibody LS-B2457 is an unconjugated goat polyclonal antibody to Pyruvate Carboxylase (PC) (aa886-899) from human. It is reactive with human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human PC / Pyruvate Carboxylase
Synonyms
PC | PCB | Pyruvate carboxylase | Pyruvic carboxylase
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
(tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-KFKEVKKAYVEANQ, from the internal region of the protein sequence according to NP_000911.2NP_001035806.1NP_071504.2.
Epitope
aa886-899
Specificity
Human PC / Pyruvate Carboxylase. All reported variants (NP_000911.2; NP_001035806.1; NP_071504.2) represent identical protein.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.03 - 0.1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B2457 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2457 was determined to be 5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PC / Pyruvate Carboxylase
Validation
Anti-Pyruvate Carboxylase antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Pyruvate Carboxylase antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ PC / Pyruvate Carboxylase Antibody (aa886-899) for IHC, WB/Western LS-B2457 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Pre-hybridisation: an efficient way of suppressing endogenous biotin-binding activity inherent to biotin-streptavidin detection system. Ahmed R, Spikings E, Zhou S, Thompsett A, Zhang T. Journal of immunological methods. 2014 Apr;406:143-7.
Customer Reviews (0)
Featured Products
Species:
Human, Mouse
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Monkey, Mouse, Rat, Bovine, Pig, Sheep, Chicken, Zebrafish
Applications:
Western blot
Species:
Human, Mouse
Applications:
IHC, Immunofluorescence, Western blot, ELISA
Reactivity:
Mouse
Range:
0.313-20 ng/ml
Reactivity:
Human
Range:
1.56-100 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
Date Requested: 4/16/2024
