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Anti-OLIG2 Antibody (aa236-285) IHC-plus™ LS-B1552

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

OLIG2 Antibody LS-B1552 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-OLIG2 antibody IHC of human brain, cortex.
Western Blot
OLIG2 antibody LS-B1552 Western blot of Jurkat lysate.
Western Blot
A: Marker, B: Jurkat Cell Lysate
 

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Rabbit Polyclonal (IgG) to Human OLIG2
Human, Chimpanzee
IHC - Paraffin, Western blot
Unconjugated

Details

Human OLIG2
Rabbit
Human, Chimpanzee (tested or 100% immunogen sequence identity)
Monkey, Mouse, Rat, Bat, Guinea pig (at least 90% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (0.2 - 1 µg/ml)

Specificity and Use

OLIG2 antibody was raised against synthetic peptide located between aa236-285 of human OLIG2 (Q13516, NP_005797). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Baboon, Monkey (100%); Galago, Marmoset, Mouse, Rat, Bat, Guinea pig, Armadillo (92%); Chicken (85%).
LS-E11520 - Lyophilized - 100 µg - $145.00
aa236-285
Human Olig2
Immunohistochemistry: LS-B1552 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1552 was determined to be 5 ug/ml.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About OLIG2

Q13516 NM_005806 NP_005797.1

OLIG2 Antibody, BHLHB1 Antibody, BHLHe19 Antibody, OLIGO2 Antibody, PRKCBP2 Antibody, RACK17 Antibody

This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome.

Immunohistochemistry

Immunohistochemistry
Anti-OLIG2 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1552 concentration 5 ug/ml.

Western Blot

Western Blot
OLIG2 antibody LS-B1552 Western blot of Jurkat lysate.

Western Blot

Western Blot
A: Marker, B: Jurkat Cell Lysate
 

Requested From: United States
Date Requested: 11/1/2014

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