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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3399-50 | 50 µg (1 mg/ml) | $460 |
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IHC‑plus™ Polyclonal Rabbit anti‑Human NOTCH2 Antibody (aa2457‑2471, IHC) LS‑B3399
IHC‑plus™ Polyclonal Rabbit anti‑Human NOTCH2 Antibody (aa2457‑2471, IHC) LS‑B3399
Note: This antibody replaces LS-C3047, LS-C9340
Antibody:
NOTCH2 Rabbit anti-Human Polyclonal (aa2457-2471) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
NOTCH2 Rabbit anti-Human Polyclonal (aa2457-2471) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Specifications
Description
NOTCH2 antibody LS-B3399 is an unconjugated rabbit polyclonal antibody to NOTCH2 (aa2457-2471) from human. It is reactive with human and monkey. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
Target
Human NOTCH2
Synonyms
NOTCH2 | AGS2 | HN2 | Notch (Drosophila) homolog 2 | Notch homolog 2 (Drosophila) | Notch 2 | Notch homolog 2 | HJCYS
Host
Rabbit
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Hamster, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Synthetic peptide from human NOTCH2. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Tamarin (100%); Mouse, Rat, Hamster, Elephant, Rabbit (93%); Bovine, Dog, Pig (87%); Panda, Bat (80%).
Epitope
aa2457-2471
Specificity
KLH conjugated synthetic peptide comprising amino acids 2457 - 2471 [THMSEPPHNNMQVYA] of the human NOTCH homolog 2 (NOTCH2) protein.
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- ELISA (1:1000)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3399 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3399 was determined to be 5 ug/ml.
Presentation
PBS, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NOTCH2
Validation
Anti-NOTCH2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NOTCH2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NOTCH2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NOTCH2 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NOTCH2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-NOTCH2 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ NOTCH2 Antibody (aa2457-2471) for IHC, ELISA LS-B3399 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Immunolocalization of notch signaling protein molecules in a maxillary chondrosarcoma and its recurrent tumor. Siar CH, Ha KO, Aung LO, Nakano K, Tsujigiwa H, Nagatsuka H, Ng KH, Kawakami T. European journal of medical research. 2010 15:456-60. (IHC-P; Human)
Notch signaling and ghost cell fate in the calcifying cystic odontogenic tumor. Siar CH, Kawakami T, Buery RR, Nakano K, Tomida M, Tsujigiwa H, Han PP, Nagatsuka H, Ng KH. European journal of medical research. 2011 16:501-6. (IHC-P; Human)
Customer Reviews (0)
Featured Products
Species:
Human, Chimpanzee, Dog
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Species:
Human
Applications:
Western blot, Immunoprecipitation
Species:
Human, Rat
Applications:
Immunofluorescence, Western blot, Immunoprecipitation
Species:
Human, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
