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Anti-NOTCH2 / NOTCH-2 Antibody (aa2396-2409) IHC-plus™ LS-B399

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

NOTCH2 / NOTCH-2 Antibody LS-B399 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-NOTCH2 antibody IHC of human kidney.
Western Blot
Western blot of anti-Notch 2 (intra) antibody shows detection of a band at ~110 kDa...
Western Blot
Anti-Notch 2 Antibody - Western Blot.
 

100% Guaranteed 100% Guaranteed Publications Publications (3)
Rabbit Polyclonal to Human NOTCH2 / NOTCH-2
Human, Chimpanzee, Dog
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human NOTCH2 / NOTCH-2
Rabbit
Human, Chimpanzee, Dog (tested or 100% immunogen sequence identity)
Polyclonal
Sterile filtered

Applications

IHC - Paraffin (1:500), Immunofluorescence, Western blot (1:400 - 1:2000), ELISA (1:30000 - 1:90000)

Specificity and Use

NOTCH2 / NOTCH-2 antibody was raised against synthetic peptide from human NOTCH2.
aa2396-2409
Amino acid residues 2396-2409 of human Notch 2 (the total protein is 2471 aa). A residue of cysteine was added to the amino terminal end to facilitate coupling.
Immunohistochemistry: LS-B399 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B399 was determined to be 1:500.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (3)

1
Differential expression of Notch receptors and their ligands in desmoplastic ameloblastoma. Siar CH, Nakano K, Han PP, Nagatsuka H, Ng KH, Kawakami T. Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology. 2010 39:552-8. (IHC-P; Human) [PubMed:20337864]
2
Notch4 overexpression in ameloblastoma correlates with the solid/multicystic phenotype. Siar CH, Nagatsuka H, Chuah KS, Rivera RS, Nakano K, Ng KH, Kawakami T. Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics. 2010 110:224-33. (IHC-P; Human) [PubMed:20659700]
3
Immunolocalization of notch signaling protein molecules in a maxillary chondrosarcoma and its recurrent tumor. Siar CH, Ha KO, Aung LO, Nakano K, Tsujigiwa H, Nagatsuka H, Ng KH, Kawakami T. European journal of medical research. 2010 15:456-60. (IHC-P; Human) [PubMed:21156405] [PMC:PMC3352190]

About NOTCH2 / NOTCH-2

Q04721 NM_024408 NP_077719.2

NOTCH2 Antibody, AGS2 Antibody, HN2 Antibody, Notch (Drosophila) homolog 2 Antibody, Notch homolog 2 (Drosophila) Antibody, Notch 2 Antibody, Notch homolog 2 Antibody, HJCYS Antibody



NOTCH2 / NOTCH-2 Antibodies (33)

Target Reactivity Applications Host Clonality Clone Format Epitope Publications
NOTCH2 / NOTCH-2 Rabbit Polyclonal Unconjugated Human IHC-P, ELISA N-Terminus No
Anti-NOTCH2 / NOTCH-2 Antibody (N-Terminus) IHC-plus™ LS-B719
IHC Image
Rabbit Polyclonal to Human NOTCH2 / NOTCH-2
Human
IHC - Paraffin, ELISA
Unconjugated
NOTCH2 / NOTCH-2 Rabbit Polyclonal Unconjugated Human, Gorilla, Gibbon, Monkey IHC-P, ELISA aa2457-2471 Yes
Anti-NOTCH2 / NOTCH-2 Antibody (aa2457-2471) IHC-plus™ LS-B3399
PublicationsPublications (2)
IHC Image
IHC Image
Rabbit Polyclonal to Human NOTCH2 / NOTCH-2
Human, Gorilla, Gibbon, Monkey
IHC - Paraffin, ELISA
Unconjugated
NOTCH2 / NOTCH-2 Rabbit Polyclonal Unconjugated Human, Mouse, Rat IHC-P, WB, ELISA aa1684-1733 No
Anti-NOTCH2 / NOTCH-2 Antibody (aa1684-1733) LS-C121043
WB Image
Rabbit Polyclonal (IgG) to Human NOTCH2 / NOTCH-2
Human, Mouse, Rat
IHC - Paraffin, Western blot, ELISA
Unconjugated
NOTCH2 / NOTCH-2 Goat Polyclonal Unconjugated Rat IHC, WB, Flo, ELISA, Neut No
Anti-NOTCH2 / NOTCH-2 Antibody LS-C150303
Goat Polyclonal (IgG) to Rat NOTCH2 / NOTCH-2
Rat
IHC, Western blot, Flow Cytometry, ELISA, Neutralization
Unconjugated
NOTCH2 / NOTCH-2 Rabbit Polyclonal Unconjugated Human IHC-P, IHC-Fr, ELISA N-Terminus No
Anti-NOTCH2 / NOTCH-2 Antibody (N-Terminus) LS-C189476
Rabbit Polyclonal (IgG) to Human NOTCH2 / NOTCH-2
Human
IHC - Paraffin, IHC - Frozen, ELISA
Unconjugated
NOTCH2 / NOTCH-2 Rabbit Polyclonal Unconjugated Human IHC, WB C-Terminus No
Anti-NOTCH2 / NOTCH-2 Antibody (C-Terminus) LS-C83738
Rabbit Polyclonal (IgG) to Human NOTCH2 / NOTCH-2
Human
IHC, Western blot
Unconjugated
NOTCH2 / NOTCH-2 Rabbit Polyclonal Unconjugated Human, Mouse IHC, WB, ELISA N-Terminus No
Anti-NOTCH2 / NOTCH-2 Antibody (N-Terminus) LS-C124296
Rabbit Polyclonal (IgG) to Human NOTCH2 / NOTCH-2
Human, Mouse
IHC, Western blot, ELISA
Unconjugated
NOTCH2 / NOTCH-2 Rabbit Polyclonal Unconjugated Human, Mouse IHC, WB, ELISA aa2396-2409 No
Anti-NOTCH2 / NOTCH-2 Antibody (aa2396-2409) LS-C124295
Rabbit Polyclonal (IgG) to Human NOTCH2 / NOTCH-2
Human, Mouse
IHC, Western blot, ELISA
Unconjugated
NOTCH2 / NOTCH-2 Rabbit Polyclonal Unconjugated Human, Gorilla, Gibbon, Monkey, Bat, Bovine, Dog IHC-P, WB, ELISA aa2396-2409 No
Anti-NOTCH2 / NOTCH-2 Antibody (aa2396-2409) LS-C9341
Rabbit Polyclonal to Human NOTCH2 / NOTCH-2
Human, Gorilla, Gibbon, Monkey, Bat, Bovine, Dog
IHC - Paraffin, Western blot, ELISA
Unconjugated
NOTCH2 / NOTCH-2 Rabbit Polyclonal Unconjugated Human, Mouse, Rat WB, ELISA aa1678-1727 No
Anti-NOTCH2 / NOTCH-2 Antibody (aa1678-1727) LS-C121096
WB Image
Rabbit Polyclonal (IgG) to Human NOTCH2 / NOTCH-2
Human, Mouse, Rat
Western blot, ELISA
Unconjugated
NOTCH2 / NOTCH-2 Rabbit Polyclonal Unconjugated Human, Mouse, Rat WB, ELISA aa1715-1764 No
Anti-NOTCH2 / NOTCH-2 Antibody (aa1715-1764) LS-C121095
WB Image
Rabbit Polyclonal (IgG) to Human NOTCH2 / NOTCH-2
Human, Mouse, Rat
Western blot, ELISA
Unconjugated
NOTCH2 / NOTCH-2 Rabbit Polyclonal Unconjugated Human, Mouse, Bovine, Dog, Pig WB Internal No
Anti-NOTCH2 / NOTCH-2 Antibody (Internal) LS-C109704
WB Image
Rabbit Polyclonal to Human NOTCH2 / NOTCH-2
Human, Mouse, Bovine, Dog, Pig
Western blot
Unconjugated
NOTCH2 / NOTCH-2 Rabbit Polyclonal Unconjugated Human WB aa1550-1600 No
Anti-NOTCH2 / NOTCH-2 Antibody (aa1550-1600) LS-C148715
WB Image
Rabbit Polyclonal to Human NOTCH2 / NOTCH-2
Human
Western blot
Unconjugated
NOTCH2 / NOTCH-2 Hamster Monoclonal HMN2-35 RPE Conjugated Mouse, Rat Flo No
Anti-NOTCH2 / NOTCH-2 Antibody [clone HMN2-35] (RPE) LS-C187539
Hamster Monoclonal [clone HMN2-35] (IgG) to Mouse NOTCH2 / NOTCH-2
Mouse, Rat
Flow Cytometry
RPE Conjugated
NOTCH2 / NOTCH-2 Rabbit Polyclonal Unconjugated Zebrafish ELISA Internal No
Anti-NOTCH2 / NOTCH-2 Antibody (Internal) LS-C210156
Rabbit Polyclonal (IgG) to Zebrafish NOTCH2 / NOTCH-2
Zebrafish
ELISA
Unconjugated
NOTCH2 / NOTCH-2 Hamster Monoclonal HMN2-35 Unconjugated Mouse, Rat Flo No
Anti-NOTCH2 / NOTCH-2 Antibody [clone HMN2-35] LS-C188663
Hamster Monoclonal [clone HMN2-35] (IgG) to Mouse NOTCH2 / NOTCH-2
Mouse, Rat
Flow Cytometry
Unconjugated
NOTCH2 / NOTCH-2 Goat Polyclonal Unconjugated Human, Gorilla, Gibbon, Monkey, Bat, Bovine, Dog, Pig, Rabbit ELISA Internal No
Anti-NOTCH2 / NOTCH-2 Antibody (Internal) LS-C55184
Goat Polyclonal to Human NOTCH2 / NOTCH-2
Human, Gorilla, Gibbon, Monkey, Bat, Bovine, Dog, Pig, Rabbit
ELISA
Unconjugated
NOTCH2 / NOTCH-2 Rabbit Polyclonal Unconjugated Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Pig, Rabbit ELISA aa2408-2424 No
Anti-NOTCH2 / NOTCH-2 Antibody (aa2408-2424) LS-C149023
Rabbit Polyclonal to Human NOTCH2 / NOTCH-2
Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Pig, Rabbit
ELISA
Unconjugated
NOTCH2 / NOTCH-2 Rabbit Monoclonal NOD-15 Unconjugated Human, Rat IF, WB, IP No
Anti-NOTCH2 / NOTCH-2 Antibody [clone NOD-15] LS-C191107
Rabbit Monoclonal [clone NOD-15] (IgG) to Human NOTCH2 / NOTCH-2
Human, Rat
Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
NOTCH2 / NOTCH-2 Sheep Polyclonal Unconjugated Mouse WB, ELISA No
Anti-NOTCH2 / NOTCH-2 Antibody LS-C144077
Sheep Polyclonal (IgG) to Mouse NOTCH2 / NOTCH-2
Mouse
Western blot, ELISA
Unconjugated
NOTCH2 / NOTCH-2 Mouse Polyclonal Unconjugated Human WB, ELISA No
Anti-NOTCH2 / NOTCH-2 Antibody LS-C126054
Mouse Polyclonal to Human NOTCH2 / NOTCH-2
Human
Western blot, ELISA
Unconjugated
NOTCH2 / NOTCH-2 Rat Monoclonal Unconjugated Human WB, ELISA aa2063-2413 No
Anti-NOTCH2 / NOTCH-2 Antibody (aa2063-2413) LS-C37773
Rat Monoclonal (IgG2a) to Human NOTCH2 / NOTCH-2
Human
Western blot, ELISA
Unconjugated
NOTCH2 / NOTCH-2 Rabbit Monoclonal 8A1 Unconjugated Human WB, IP No
Anti-NOTCH2 / NOTCH-2 Antibody [clone 8A1] LS-C73704
Rabbit Monoclonal [clone 8A1] (IgG) to Human NOTCH2 / NOTCH-2
Human
Western blot, Immunoprecipitation
Unconjugated
NOTCH2 / NOTCH-2 Rat Monoclonal 11D93 PE Conjugated Mouse Flo aa1-528 No
Anti-NOTCH2 / NOTCH-2 Antibody (aa1-528) [clone 11D93] (PE) LS-C184012
Rat Monoclonal [clone 11D93] (IgG1) to Mouse NOTCH2 / NOTCH-2
Mouse
Flow Cytometry
PE Conjugated
NOTCH2 / NOTCH-2 Hamster Monoclonal 12G37 Unconjugated Mouse, Rat Flo No
Anti-NOTCH2 / NOTCH-2 Antibody [clone 12G37] LS-C182623
Hamster Monoclonal [clone 12G37] (IgG) to Mouse NOTCH2 / NOTCH-2
Mouse, Rat
Flow Cytometry
Unconjugated
NOTCH2 / NOTCH-2 Rabbit Polyclonal Unconjugated Human, Gorilla, Gibbon, Monkey ELISA aa2457-2471 No
Anti-NOTCH2 / NOTCH-2 Antibody (aa2457-2471) LS-C9340
Rabbit Polyclonal (IgG) to Human NOTCH2 / NOTCH-2
Human, Gorilla, Gibbon, Monkey
ELISA
Unconjugated
NOTCH2 / NOTCH-2 Rabbit Polyclonal Unconjugated Human, Gorilla, Gibbon, Monkey, Bat, Bovine, Dog ELISA aa2396-2409 No
Anti-NOTCH2 / NOTCH-2 Antibody (aa2396-2409) LS-C73702
Rabbit Polyclonal (IgG) to Human NOTCH2 / NOTCH-2
Human, Gorilla, Gibbon, Monkey, Bat, Bovine, Dog
ELISA
Unconjugated
NOTCH2 / NOTCH-2 Hamster Monoclonal 12G36 Unconjugated Mouse, Rat Flo No
Anti-NOTCH2 / NOTCH-2 Antibody [clone 12G36] LS-C182622
Hamster Monoclonal [clone 12G36] (IgG) to Mouse NOTCH2 / NOTCH-2
Mouse, Rat
Flow Cytometry
Unconjugated
NOTCH2 / NOTCH-2 Goat Polyclonal APC Conjugated Rat Flo aa26-492 No
Anti-NOTCH2 / NOTCH-2 Antibody (aa26-492) (APC) LS-C126056
Goat Polyclonal (IgG) to Rat NOTCH2 / NOTCH-2
Rat
Flow Cytometry
APC Conjugated
NOTCH2 / NOTCH-2 Goat Polyclonal PE Conjugated Rat Flo aa26-492 No
Anti-NOTCH2 / NOTCH-2 Antibody (aa26-492) (PE) LS-C126055
Goat Polyclonal (IgG) to Rat NOTCH2 / NOTCH-2
Rat
Flow Cytometry
PE Conjugated
NOTCH2 / NOTCH-2 Rabbit Polyclonal Unconjugated Zebrafish, Fish ELISA No
Anti-NOTCH2 / NOTCH-2 Antibody LS-C182363
Rabbit Polyclonal (IgG) to Zebrafish NOTCH2 / NOTCH-2
Zebrafish, Fish
ELISA
Unconjugated
NOTCH2 / NOTCH-2 Goat Polyclonal CF Conjugated Rat Flo aa26-492 No
Anti-NOTCH2 / NOTCH-2 Antibody (aa26-492) (CF) LS-C126057
Goat Polyclonal (IgG) to Rat NOTCH2 / NOTCH-2
Rat
Flow Cytometry
CF Conjugated
NOTCH2 / NOTCH-2 Rabbit Polyclonal Unconjugated Human, Chimpanzee, Dog IHC-P, IF, WB, ELISA aa2396-2409 Yes
Anti-NOTCH2 / NOTCH-2 Antibody (aa2396-2409) IHC-plus™ LS-B399
PublicationsPublications (3)
IHC Image
WB Image
Rabbit Polyclonal to Human NOTCH2 / NOTCH-2
Human, Chimpanzee, Dog
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Immunohistochemistry

Immunohistochemistry
Anti-NOTCH2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B399 dilution 1:500.

Western Blot

Western Blot
Western blot of anti-Notch 2 (intra) antibody shows detection of a band at ~110 kDa corresponding to active Notch 2 protein (arrowhead). Western Blot analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (u

Western Blot

Western Blot
Anti-Notch 2 Antibody - Western Blot. Western blot of anti-Notch 2 (intra) antibody shows detection of a band at ~110 kD corresponding to active Notch 2 protein (arrowhead). Western Blot analysis was performed for Notch 2 expression using 100 ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.
 

Requested From: United States
Date Requested: 4/19/2014
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