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| Catalog Number | Size | Price |
|---|---|---|
| LS-B3584-50 | 50 µl (1 mg/ml) | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human MYL2 Antibody (clone AT3B2, IHC, WB) LS‑B3584
IHC‑plus™ Monoclonal Mouse anti‑Human MYL2 Antibody (clone AT3B2, IHC, WB) LS‑B3584
Note: This antibody replaces LS-C103487
Antibody:
MYL2 Mouse anti-Human Monoclonal (AT3B2) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
MYL2 Mouse anti-Human Monoclonal (AT3B2) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Specifications
Description
MYL2 antibody LS-B3584 is an unconjugated mouse monoclonal antibody to MYL2 from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human MYL2
Synonyms
MYL2 | MLC2 | Myosin light chain 2 | MLC-2 | RLC of myosin | CMH10 | MLC-2v
Host
Mouse
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG2b,k
Monoclonal
Clone
AT3B2
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Recombinant human MYL2 (1-166aa) purified from E. coli
Specificity
human MYL2 (1-166aa)
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (1:1000 - 1:2000)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B3584 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3584 was determined to be 5 ug/ml. Western Blot: Extracts of Rat heart (60ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human MYL2 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About MYL2
Validation
Anti-MYL2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-MYL2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ MYL2 Antibody (clone AT3B2) for IHC, WB/Western, ELISA LS-B3584 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
![MYL2 Antibody - Immunofluorescence of monoclonal antibody to MYL2 on HeLa cell . [antibody concentration 10 ug/ml]](https://lsbio-7d62.kxcdn.com//image2/myl2-antibody-clone-3b9-b4-ls-c197730/145738_974559.jpg)
Species:
Human
Applications:
Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Source:
Human
Tag:
Myc-DDK (Flag)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/17/2024
Date Requested: 4/17/2024
