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Anti-MLH1 Antibody IHC-plus™ LS-B3475

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

MLH1 Antibody LS-B3475 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-MLH1 antibody IHC of human small intestine.

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Mouse Monoclonal (IgG1) to Human MLH1
Human, Monkey
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human MLH1
Mouse
Human, Monkey (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Ascites

Applications

IHC - Paraffin (1:200), Immunofluorescence (1:200 - 1:1000), Western blot (1:500 - 1:2000), ELISA (1:10000)

Specificity and Use

MLH1 antibody was raised against purified recombinant fragment of MLH1 (aa381-483) expressed in E. coli.
Immunohistochemistry: LS-B3475 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3475 was determined to be 1:200.

Packaging

Ascites fluid, 0.03% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About MLH1

P40692 NM_000249 NP_000240.1

MLH1 Antibody, COCA2 Antibody, FCC2 Antibody, HMLH1 Antibody, HNPCC Antibody, HNPCC2 Antibody, MutL protein homolog 1 Antibody

MLH1 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.


MLH1 Antibodies (28)

Target Reactivity Applications Host Clonality Clone Format Epitope Publications
MLH1 Rabbit Monoclonal EPR3894 Unconjugated Human, Mouse, Rat IHC-P, WB, IP, Flo Internal No
Anti-MLH1 Antibody (Internal) [clone EPR3894] LS-C105712
IHC Image
IHC Image
Rabbit Monoclonal [clone EPR3894] (IgG) to Human MLH1
Human, Mouse, Rat
IHC - Paraffin, Western blot, Immunoprecipitation, Flow Cytometry
Unconjugated
MLH1 Mouse Monoclonal G168-15 Unconjugated Human, Rat, Hamster IHC-P No
Anti-MLH1 Antibody [clone G168-15] LS-C95607
Mouse Monoclonal [clone G168-15] (IgG1)
Human, Rat, Hamster
IHC - Paraffin
Unconjugated
MLH1 Rabbit Polyclonal Unconjugated Human IHC-P Internal No
Anti-MLH1 Antibody (Internal) LS-C210492
IHC Image
Rabbit Polyclonal (IgG) to Human MLH1
Human
IHC - Paraffin
Unconjugated
MLH1 Rabbit Polyclonal Unconjugated Human IHC-P, IHC-Fr, WB, IP No
Anti-MLH1 Antibody LS-C43060
WB Image
Rabbit Polyclonal (IgG) to Human MLH1
Human
IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated
MLH1 Mouse Monoclonal Unconjugated Human IHC-P, IHC-Fr No
Anti-MLH1 Antibody LS-C9060
Mouse Monoclonal (IgG1,k) to Human MLH1
Human
IHC - Paraffin, IHC - Frozen
Unconjugated
MLH1 Rabbit Polyclonal Unconjugated Human IHC-Fr, WB, IP No
Anti-MLH1 Antibody LS-C9064
Rabbit Polyclonal to Human MLH1
Human
IHC - Frozen, Western blot, Immunoprecipitation
Unconjugated
MLH1 Mouse Monoclonal Unconjugated Human, Mouse, Rat IHC-P, ICC, IF No
Anti-MLH1 Antibody LS-C72708
Mouse Monoclonal (IgG1) to Human MLH1
Human, Mouse, Rat
IHC - Paraffin, ICC, Immunofluorescence
Unconjugated
MLH1 Mouse Monoclonal M1 Unconjugated Human IF, WB, ELISA No
Anti-MLH1 Antibody [clone M1] LS-C133324
IF Image
WB Image
Mouse Monoclonal [clone M1] (IgG) to Human MLH1
Human
Immunofluorescence, Western blot, ELISA
Unconjugated
MLH1 Rabbit Polyclonal Unconjugated Human ICC, IF, WB No
Anti-MLH1 Antibody LS-C109333
IF Image
WB Image
Rabbit Polyclonal to Human MLH1
Human
ICC, Immunofluorescence, Western blot
Unconjugated
MLH1 Rabbit Polyclonal Unconjugated Human WB, Flo aa459-488 No
Anti-MLH1 Antibody (aa459-488) LS-C164215
Flo Image
WB Image
Rabbit Polyclonal to Human MLH1
Human
Western blot, Flow Cytometry
Unconjugated
MLH1 Rabbit Polyclonal Unconjugated Human, Mouse, Rat WB, ELISA aa441-490 No
Anti-MLH1 Antibody (aa441-490) LS-C119574
WB Image
Rabbit Polyclonal (IgG) to Human MLH1
Human, Mouse, Rat
Western blot, ELISA
Unconjugated
MLH1 Rabbit Monoclonal EPR3893 Unconjugated Human, Mouse, Rat ICC, WB Internal No
Anti-MLH1 Antibody (Internal) [clone EPR3893] LS-C138000
WB Image
Rabbit Monoclonal [clone EPR3893] to Human MLH1
Human, Mouse, Rat
ICC, Western blot
Unconjugated
MLH1 Rabbit Polyclonal Unconjugated Human, Mouse, Rat IF, WB Ser467 No
Anti-MLH1 Antibody (Ser467) LS-C176980
WB Image
Rabbit Polyclonal to Human MLH1
Human, Mouse, Rat
Immunofluorescence, Western blot
Unconjugated
MLH1 Mouse Monoclonal 164C819 Unconjugated Human, Gorilla, Monkey WB aa387-403 Yes
Anti-MLH1 Antibody (aa387-403) [clone 164C819] LS-C566
PublicationsPublications (1)
WB Image
Mouse Monoclonal [clone 164C819] (IgG) to Human MLH1
Human, Gorilla, Monkey
Western blot
Unconjugated
MLH1 Mouse Monoclonal Unconjugated Human WB No
Anti-MLH1 Antibody LS-C158152
WB Image
Mouse Monoclonal (IgG1) to Human MLH1
Human
Western blot
Unconjugated
MLH1 Rabbit Polyclonal Unconjugated Human, Mouse, Rat, Dog, Horse, Pig, Rabbit, Xenopus WB N-Terminus No
Anti-MLH1 Antibody (N-Terminus) LS-C111793
WB Image
Rabbit Polyclonal to Human MLH1
Human, Mouse, Rat, Dog, Horse, Pig, Rabbit, Xenopus
Western blot
Unconjugated
MLH1 Rabbit Polyclonal Unconjugated Human, Gorilla, Gibbon, Monkey, Dog, Horse, Chicken, Xenopus ELISA aa381-395 No
Anti-MLH1 Antibody (aa381-395) LS-C148019
Rabbit Polyclonal to Human MLH1
Human, Gorilla, Gibbon, Monkey, Dog, Horse, Chicken, Xenopus
ELISA
Unconjugated
MLH1 Rabbit Polyclonal Unconjugated Human, Gorilla ELISA aa470-489 No
Anti-MLH1 Antibody (aa470-489) LS-C148018
Rabbit Polyclonal to Human MLH1
Human, Gorilla
ELISA
Unconjugated
MLH1 Rabbit Polyclonal FITC Conjugated Human WB, Flo, ELISA aa459-488 No
Anti-MLH1 Antibody (aa459-488) (FITC) LS-C263231
Rabbit Polyclonal (IgG) to Human MLH1
Human
Western blot, Flow Cytometry, ELISA
FITC Conjugated
MLH1 Rabbit Polyclonal HRP Conjugated Human WB, Flo, ELISA aa459-488 No
Anti-MLH1 Antibody (aa459-488) (HRP) LS-C263234
Rabbit Polyclonal (IgG) to Human MLH1
Human
Western blot, Flow Cytometry, ELISA
HRP Conjugated
MLH1 Rabbit Polyclonal PE Conjugated Human WB, Flo, ELISA aa459-488 No
Anti-MLH1 Antibody (aa459-488) (PE) LS-C263235
Rabbit Polyclonal (IgG) to Human MLH1
Human
Western blot, Flow Cytometry, ELISA
PE Conjugated
MLH1 Rabbit Polyclonal APC Conjugated Human WB, Flo, ELISA aa459-488 No
Anti-MLH1 Antibody (aa459-488) (APC) LS-C263228
Rabbit Polyclonal (IgG) to Human MLH1
Human
Western blot, Flow Cytometry, ELISA
APC Conjugated
MLH1 Rabbit Polyclonal Biotin Conjugated Human WB, Flo, ELISA aa459-488 No
Anti-MLH1 Antibody (aa459-488) (Biotin) LS-C263229
Rabbit Polyclonal (IgG) to Human MLH1
Human
Western blot, Flow Cytometry, ELISA
Biotin Conjugated
MLH1 Rabbit Polyclonal AP Conjugated Human WB, Flo, ELISA aa459-488 No
Anti-MLH1 Antibody (aa459-488) (AP) LS-C263230
Rabbit Polyclonal (IgG) to Human MLH1
Human
Western blot, Flow Cytometry, ELISA
AP Conjugated
MLH1 Rabbit Polyclonal Unconjugated Human WB, IP aa633-662 No
Anti-MLH1 Antibody (aa633-662) LS-C72710
Rabbit Polyclonal to Human MLH1
Human
Western blot, Immunoprecipitation
Unconjugated
MLH1 Rabbit Polyclonal Unconjugated Human WB aa557-756 No
Anti-MLH1 Antibody (aa557-756) LS-C189102
Rabbit Polyclonal to Human MLH1
Human
Western blot
Unconjugated
MLH1 Rabbit Polyclonal Unconjugated Human IP No
Anti-MLH1 Antibody LS-C72711
Rabbit Polyclonal to Human MLH1
Human
Immunoprecipitation
Unconjugated
MLH1 Mouse Monoclonal Unconjugated Human, Monkey IHC-P, IF, WB, ELISA No
Anti-MLH1 Antibody IHC-plus™ LS-B3475
IHC Image
Mouse Monoclonal (IgG1) to Human MLH1
Human, Monkey
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Immunohistochemistry

Immunohistochemistry
Anti-MLH1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3475 dilution 1:200.
 

Requested From: United States
Date Requested: 4/19/2014
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