Human, Monkey (tested or 100% immunogen sequence identity)
IHC - Paraffin (1:200), Immunofluorescence (1:200 - 1:1000), Western blot (1:500 - 1:2000), ELISA (1:10000)
Specificity and Use
MLH1 antibody was raised against purified recombinant fragment of MLH1 (aa381-483) expressed in E. coli.
Immunohistochemistry: LS-B3475 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3475 was determined to be 1:200.
Ascites fluid, 0.03% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
MLH1 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC.