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Anti-MIF Antibody (clone 4E4) IHC-plus™ LS-B2925

See our new MIF ELISA Kits
Human MIF ELISA Kit - LS-F152
Human MIF ELISA Kit - LS-F712
(kits do not necessarily contain the antibody listed on this page)

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

MIF Antibody LS-B2925 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-MIF antibody IHC of human breast.
Western Blot
The extract of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with...

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Mouse Monoclonal [clone 4E4] (IgG1,k) to Human MIF
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human MIF
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [4E4]
Protein G purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (1:500 - 1:2000), ELISA

Specificity and Use

MIF antibody was raised against recombinant human MIF (1-114 aa) purified from E. coli.
Immunohistochemistry: LS-B2925 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2925 was determined to be 5 ug/ml.

Packaging

PBS, pH 7.4, 0.1% sodium azide. Sourced in Ascites.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About MIF

P14174 NM_002415 NP_002406.1

MIF Antibody, GLIF Antibody, L-dopachrome tautomerase Antibody, L-dopachrome isomerase Antibody, MMIF Antibody, Phenylpyruvate tautomerase Antibody


Immunohistochemistry

Immunohistochemistry
Anti-MIF antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2925 concentration 5 ug/ml.

Western Blot

Western Blot
The extract of HL-60 was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MIF antibody (1:1000). Protein was visualized using a goat anti-mouse secondary antibody conjugated to HRP and aN ECL detection system.

Requested From: United States
Date Requested: 10/20/2014

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