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Anti-MCM2 Antibody (aa21-31) IHC-plus™ LS-B391

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

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MCM2 Antibody LS-B391 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-MCM2 antibody IHC of human skin.
Western Blot
Anti-MCM2 Antibody - Western Blot.

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Rabbit Polyclonal to Human MCM2
Human, Mouse, Rat, Yeast
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated

Details

Human MCM2
Rabbit
Human, Mouse, Rat, Yeast (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Immunofluorescence, Western blot (1:500 - 1:2000), ELISA (1:10000 - 1:50000)

Specificity and Use

MCM2 antibody was raised against synthetic peptide from human MCM2.
aa21-31
Amino acids 21-31 of human MCM2 protein (see below).
Immunohistochemistry: LS-B391 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B391 was determined to be 5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Aliquot and store at -20°C. Minimize freezing and thawing.
For research use only.

Publications (1)

1
The opposing transcriptional functions of Sin3a and c-Myc are required to maintain tissue homeostasis. Nascimento EM, Cox CL, MacArthur S, Hussain S, Trotter M, Blanco S, Suraj M, Nichols J, Kbler B, Benitah SA, Hendrich B, Odom DT, Frye M. Nature cell biology. 2011 13:1395-405. (IHC-P; Mouse) [PubMed:22101514] [PMC:PMC3242072]

About MCM2

P49736 NM_004526 NP_004517.2

MCM2 Antibody, BM28 Antibody, Cell devision cycle-like 1 Antibody, D3S3194 Antibody, Cdc19 Antibody, Cyclin-like 1 Antibody, KIAA0030 Antibody, Nuclear protein BM28 Antibody, CDCL1 Antibody, MITOTIN Antibody

MCM2, also called CDCL1 and BM28, is a nuclear protein that allows DNA to undergo a single round of replication per cell cycle. It is required for the entry in S phase and for cell division. Increasing levels of MCM2 have been linked to increasing risk levels in some types of carcinoma.

Immunohistochemistry

Immunohistochemistry
Anti-MCM2 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B391 concentration 5 ug/ml.

Western Blot

Western Blot
Anti-MCM2 Antibody - Western Blot. Western blot of Affinity Purified anti-MCM2 antibody shows detection of both phosphorylated and unphosphorylated MCM2 present in nuclear extracts from elutriated human cells (MO59K/K562). The MCM2 protein is phosphorylated after initiation of DNA replication, therefore, the protein is unphosphorylated in early S phase, and gradually becomes phosphorylated throughout S phase. In G2/M, all MCM2 is phosphorylated. Panel A shows western blot results for lysates were prepared from asynchronous cells (lane 1), cells arrested in early S with aphidicolin (lane2), and cells arrested in mitosis with nocodazole (lane 3). Panel B shows a schematic diagram of bands representing phosphorylated and unphosphorylated MCM2 present in these preparations. Asynchronous cells contain a doublet of both forms. Aphidicolin treated cells contain only unphosphorylated MCM2 and nocodozole treatment results in only phosphorylated MCM2 detected in the lysate. The phosphorylated band migrates faster than the unphosphorylated form and is seen as the lower band. There is a clear switch from the unphosphorylated form in the center lane, to the phosphorylated form in the third lane, confirming recognition of both forms of MCM2 by this antibody. The primary antibody was diluted 1:400 for this experiment. Personal Communication, Jennifer Seiler, NIH, CCR, Bethesda, MD.

Requested From: United States
Date Requested: 9/20/2014

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