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Anti-MAP2K1 / MEK1 Antibody (N-Terminus) IHC-plus™ LS-B3271

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

MAP2K1 / MEK1 Antibody LS-B3271 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-MAP2K1 / MEK1 antibody IHC of human prostate.
Immunohistochemistry
Anti-MAP2K1 / MEK1 antibody IHC of human tonsil.
Immunohistochemistry
Anti-MAP2K1 / MEK1 antibody IHC of human brain, cerebellum.
Immunofluorescence
Confocal immunofluorescent staining of HeLa cells using LS-B3271(green); nuclei are stained in...
Western Blot
Western blot of human thymus HS67 cell lysate.
 

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Rabbit Polyclonal to Human MAP2K1 / MEK1
Human, Mouse, Rat, Xenopus
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human MAP2K1 / MEK1
Rabbit
Human, Mouse, Rat, Xenopus (tested or 100% immunogen sequence identity)
Polyclonal
Protein A purified

Applications

IHC - Paraffin (10 µg/ml), Immunofluorescence, Western blot (1 µg/ml)

Specificity and Use

MAP2K1 / MEK1 antibody was raised against synthetic peptide derived from sequence near the amino-terminus of human MEK1, conjugated to KLH.
N-Terminus
Immunohistochemistry: LS-B3271 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3271 was determined to be 10 ug/ml.

Packaging

PBS, pH 7.2, 50% glycerol, 0.09% azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About MAP2K1 / MEK1

Q02750 NM_002755 NP_002746.1

MAP2K1 Antibody, ERK activator kinase 1 Antibody, MAPK/ERK kinase 1 Antibody, MAPKK 1 Antibody, MKK1 Antibody, PRKMK1 Antibody, MEK 1 Antibody, MAP kinase kinase 1 Antibody, MAPKK1 Antibody, MEK1 Antibody

MEK1, a MAP2K type protein kinase, is a member of the Ras MAPK pathway. This pathway modulates the activity of transcription factors, structural proteins and other protein kinases, leading to biological responses such as cell proliferation and differentiation. MEK1 contains a proline-rich segment that is recognized by the scaffold protein MEK binding partner-1 (MP1). MP1 associates with non-phosphorylated MEK1, forming a complex which associates with and phosphorylates MAP kinases ERK1 and 2.

Immunohistochemistry

Immunohistochemistry
Anti-MAP2K1 / MEK1 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3271 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-MAP2K1 / MEK1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3271 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-MAP2K1 / MEK1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3271 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence
Confocal immunofluorescent staining of HeLa cells using LS-B3271(green); nuclei are stained in blue pseudocolor using DRAQ5.

Western Blot

Western Blot
Western blot of human thymus HS67 cell lysate.
 

Requested From: United States
Date Requested: 7/24/2014
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