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Anti-LMNA / Lamin A/C Antibody (C-Terminus) IHC-plus™ LS-B2005

Ordering

Wt. Vol. Conc. Price
- 50 µl - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

LMNA / Lamin A/C Antibody LS-B2005 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-Lamin A/C antibody IHC of human skin.
Western Blot
Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and...

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Rabbit Polyclonal (IgG) to Mouse LMNA / Lamin A/C
Mouse, Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Mouse LMNA / Lamin A/C
Rabbit
Mouse, Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (1:50), Immunofluorescence, Western blot

Specificity and Use

LMNA / Lamin A/C antibody was raised against recombinant mouse LMNA / Lamin A.
C-Terminus
Recombinant (partial), C-terminal. The immunogen is taken from isoform C and may not cross-react with isoform A.
Immunohistochemistry: LS-B2005 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2005 was determined to be 1:50.

Packaging

Phosphate-buffered solution, pH 7.2, 0.09% sodium azide, 50% glycerol.
+4°C or -20°C, Avoid repeated freezing and thawing.
For research use only.

Publications (2)

1
Pim-1 kinase-dependent phosphorylation of p21Cip1/WAF1 regulates its stability and cellular localization in H1299 cells. Zhang Y, Wang Z, Magnuson NS. Molecular cancer research : MCR. 2007 5:909-22. [PubMed:17855660]
2
Chronic mucocutaneous candidiasis caused by a gain-of-function mutation in the STAT1 DNA-binding domain. Takezaki S, Yamada M, Kato M, Park MJ, Maruyama K, Yamazaki Y, Chida N, Ohara O, Kobayashi I, Ariga T. Journal of immunology (Baltimore, Md. : 1950). 2012 189:1521-6. [PubMed:22730530]

About LMNA / Lamin A/C

P02545 NM_005572 NP_005563.1

LMNA Antibody, 70 kDa lamin Antibody, CDCD1 Antibody, CMD1A Antibody, EMD2 Antibody, FPLD Antibody, IDC Antibody, FPL Antibody, FPLD2 Antibody, Lamin A/C Antibody, Lamin Antibody, LFP Antibody, LMNC Antibody, LMN1 Antibody, LMNL1 Antibody, HGPS Antibody, Prelamin-A/C Antibody, PRO1 Antibody, Lamin A Antibody, Lamin A/C-like 1 Antibody, LGMD1B Antibody, CDDC Antibody, CMT2B1 Antibody, LDP1 Antibody


Immunohistochemistry

Immunohistochemistry
Anti-Lamin A/C antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2005 dilution 1:50.

Western Blot

Western Blot
Hela cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit polyclonal anti-lamin A. Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence system.

Requested From: United States
Date Requested: 7/28/2014
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