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Anti-IRAK4 / IRAK-4 Antibody (clone 2H9) IHC-plus™ LS-B10452

Ordering

Wt. Vol. Conc. Price
- 100 µl - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

IRAK4 / IRAK-4 Antibody LS-B10452 Images

[click image for a larger image and more details]
Immunohistochemistry
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunohistochemistry
IHC of paraffin-embedded human lung cancer tissues using IRAK4 mouse mAb with DAB staining.
Immunohistochemistry
IHC of paraffin-embedded human kidney cancer tissues using IRAK4 mouse mAb with DAB staining.
Flow Cytometry
Flow cytometry of HeLa cells using IRAK4 mouse mAb (blue) and negative control (red).
Western Blot
Western blot of IRAK4 mouse mAb against THP-1 (1), HeLa (2), K562 (3), MCF-7 (4), RAW264.7 (5),...
Western Blot
Western blot of IRAK4 mAb against human IRAK4 (AA: 21-198) recombinant protein.
ELISA
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
 

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 2H9] (IgG1) to Human IRAK4 / IRAK-4
Human, Monkey, Mouse
IHC, IHC - Paraffin, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human IRAK4 / IRAK-4
Mouse
Human, Monkey, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [2H9]
Ascites

Applications

IHC (1:200 - 1:1000), IHC - Paraffin (1:50), Western blot (1:500 - 1:2000), Flow Cytometry (1:200 - 1:400), ELISA (1:10000)

Specificity and Use

IRAK4 / IRAK-4 antibody was raised against purified recombinant fragment of human IRAK4 expressed in E. coli.
Human IRAK4

Packaging

Ascites, 0.03% sodium azide
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About IRAK4 / IRAK-4

Q9NWZ3 NM_016123 NP_057207.2

IRAK4 Antibody, IPD1 Antibody, IRAK-4 Antibody, NY-REN-64 Antibody, REN64 Antibody

IRAK4, an IRAK type protein kinase, is a signal transducer for the immune response Toll-like receptor and interleukin-1 (IL-1) receptor signaling cascades. IRAK4 has auto- and cross-phosphorylation kinase activity and has been shown to phosphorylate and activate IRAK1. Additionally, IRAK4 interacts with IRAK1 and TRAF6 in an IL-1-dependent manner, and overexpression of IRAK4 can activate NF-kappaB as well as mitogen-activated protein (MAP) kinase pathways.

Immunohistochemistry

Immunohistochemistry
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded human lung cancer tissues using IRAK4 mouse mAb with DAB staining.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded human kidney cancer tissues using IRAK4 mouse mAb with DAB staining.

Flow Cytometry

Flow Cytometry
Flow cytometry of HeLa cells using IRAK4 mouse mAb (blue) and negative control (red).

Western Blot

Western Blot
Western blot of IRAK4 mouse mAb against THP-1 (1), HeLa (2), K562 (3), MCF-7 (4), RAW264.7 (5), Jurkat (6) and Cos7 (7) cell lysate.

Western Blot

Western Blot
Western blot of IRAK4 mAb against human IRAK4 (AA: 21-198) recombinant protein. (Expected MW is 45.4 kDa)

ELISA

ELISA
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
 

Requested From: United States
Date Requested: 10/1/2014

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