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Anti-IRAK4 / IRAK-4 Antibody (aa38-54) IHC-plus™ LS-B2048

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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IRAK4 / IRAK-4 Antibody LS-B2048 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-IRAK4 antibody IHC of human kidney.
Western Blot
Western blot of IRAK-4 in 30 ugs of NIH 3T3 cell lysate using antibody at 1:500 dilution.

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Rabbit Polyclonal (IgG) to Mouse IRAK4 / IRAK-4
Mouse, Human
IHC - Paraffin, Western blot
Unconjugated

Details

Mouse IRAK4 / IRAK-4
Rabbit
Mouse, Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Protein G purified

Applications

IHC - Paraffin (10 µg/ml), Western blot (1:500 - 1:1000)

Specificity and Use

IRAK4 / IRAK-4 antibody was raised against synthetic peptide from mouse IRAK4.
aa38-54
A mixture of synthetic peptides containing amino acids 38-54 and 120-136 of mouse IRAK-4.
Immunohistochemistry: LS-B2048 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2048 was determined to be 10 ug/ml.

Packaging

PBS, 0.05% BSA, 0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About IRAK4 / IRAK-4

Q9NWZ3 NM_016123 NP_057207.2

IRAK4 Antibody, IPD1 Antibody, IRAK-4 Antibody, NY-REN-64 Antibody, REN64 Antibody

IRAK4, an IRAK type protein kinase, is a signal transducer for the immune response Toll-like receptor and interleukin-1 (IL-1) receptor signaling cascades. IRAK4 has auto- and cross-phosphorylation kinase activity and has been shown to phosphorylate and activate IRAK1. Additionally, IRAK4 interacts with IRAK1 and TRAF6 in an IL-1-dependent manner, and overexpression of IRAK4 can activate NF-kappaB as well as mitogen-activated protein (MAP) kinase pathways.

Immunohistochemistry

Immunohistochemistry
Anti-IRAK4 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2048 concentration 10 ug/ml.

Western Blot

Western Blot
Western blot of IRAK-4 in 30 ugs of NIH 3T3 cell lysate using antibody at 1:500 dilution.

Requested From: United States
Date Requested: 9/2/2014

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