Human IL6 / Interleukin 6

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Antiserum

Unmodified

This whole rabbit serum was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli.

• IHC (1:400 - 1:800)
• Western blot (1:500 - 1:2000)
• Immunoprecipitation (1:400 - 1:800)
• ELISA (1:1000 - 1:5000)
• Neutralization (1:400)
• Radioimmunoassay

This antiserum against IL-6 has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation and immunoblotting. Reactivity in other immunoassays is unknown. For immunoblotting use the supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serum plus 10 ng/mL E. coli LPS. For immunohistochemistry either paraffin fix or cryofix tissue. For immunoprecipitation, pre-clearing with a non-specific rabbit IgG is helpful to reduce background.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl

Short term: -20°C; Long term: -20°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P05231 NCBI: NM_000600 NP_000591.1