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Anti-IL13 Antibody IHC-plus™ LS-B7417

See our new IL13 ELISA Kits
Human IL13 ELISA Kit - LS-F111
Mouse IL13 ELISA Kit - LS-F255
(kits do not necessarily contain the antibody listed on this page)

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl - $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

IL13 Antibody LS-B7417 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-IL-13 antibody IHC of human tonsil, germinal center.
Western Blot
Western Blot (non-reducing) of IL-13 antibody LS-B7417
Western Blot
Western Blot (reducing) of IL-13 antibody LS-B7417
ELISA
Sandwich ELISA of IL-13 antibody LS-B7417

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Rabbit Polyclonal (IgG) to Human IL13
Human
IHC - Paraffin, Western blot, ELISA, Neutralization
Unconjugated

Details

Human IL13
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (0.1 - 0.2 µg/ml), ELISA (0.5 - 2 µg/ml), Neutralization (0.12 - 0.15 µg/ml)

Specificity and Use

IL13 antibody was raised against e.coli derived recombinant Human IL-13.
Human IL-13
Immunohistochemistry: LS-B7417 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hIL-13 (2 ng/ml), a concentration of 0.12-0.15 ug/ml of this antibody is required. ELISA: To detect hIL-13 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human IL-13 (LS-C104340) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-13. Western Blot: To detect hIL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-13 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
50 µl Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About IL13

P35225 NM_002188 NP_002179.2

IL13 Antibody, Allergic rhinitis Antibody, ALRH Antibody, BHR1 Antibody, Interleukin-13 Antibody, NC30 Antibody, p600 Antibody, IL-13 Antibody, Interleukin 13 Antibody


Immunohistochemistry

Immunohistochemistry
Anti-IL-13 antibody IHC of human tonsil, germinal center. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7417 concentration 5 ug/ml.

Western Blot

Western Blot
Western Blot (non-reducing) of IL-13 antibody LS-B7417

Western Blot

Western Blot
Western Blot (reducing) of IL-13 antibody LS-B7417

ELISA

ELISA
Sandwich ELISA of IL-13 antibody LS-B7417

Requested From: United States
Date Requested: 8/31/2014

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