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Catalog Number Size Price
LS-C60602-2 2 mg (2 mg/ml) $342 
Human IgG Antibody - ELISA results of Rabbit anti-Human IgG Antibody Peroxidase Conjugated tested against purified Human IgG protein. Each well was coated in duplicate with 1.0 µg of Human IgG  The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate

Polyclonal Rabbit Anti‑Human IgG Secondary Antibody (HRP, IHC, WB) LS‑C60602

Polyclonal Rabbit Anti‑Human IgG Secondary Antibody (HRP, IHC, WB) LS‑C60602

Antibody:
Human IgG Rabbit anti-Human Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA, DB, EM
Reactivity:
Human
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$342
LS-C60602-2
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Human IgG Rabbit anti-Human Polyclonal (HRP) Antibody
Application:
IHC, WB, ELISA, DB, EM
Reactivity:
Human
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
Human IgG antibody LS-C60602 is an HRP-conjugated rabbit polyclonal antibody. Validated for DB, ELISA, EM, IHC and WB.
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Anti-Human IgG whole molecule was produced by repeated immunization with Human IgG whole molecule in rabbit.
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG and Human Serum.
Applications
  • IHC (1:1000 - 1:5000)
  • Western blot (1:5000 - 1:25000)
  • ELISA (1:150000 - 1:250000)
  • Dot Blot
  • Electron Microscopy
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Storage
Store at 4°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024